FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421638
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45470.31984232272913354No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38540.2710957360453224No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTCCAC37070.2607555509911806No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35030.24640590642624913No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTCCACAACACTCAGG33140.23311138278520974No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31780.22354495307525543No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31350.22052027309343164No Hit
GCCTGGGATAGCAGCCTGAGTGTTGTGGAATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG31070.21855071403549992No Hit
GTAATATACAGCCGTGTCCTCCTCTCTCAGGCTATTCATTTGCAAATACA28760.2023018518075628No Hit
ATCCCAGGCAGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG28480.20033229274963107No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24800.17444665941681356No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC24310.170999931065433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24060.1692413961922796No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22980.16164452554025707No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG22830.16058940461636506No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22790.16030803903666055No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21630.15214843722522894No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGAA21080.14827966050429153No Hit
GTATATTACTGTGTGAGATTGAGGGGTCCTGCAATGAGTACGATTTTCTT20790.14623976005143363No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20690.14553634610217228No Hit
GAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACC20620.14504395633768935No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20570.14469224936305866No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20390.14342610425438826No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19800.1392759619537463No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19760.1389945963740418No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGC19040.13393001593936008No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18600.1308349945626102No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18580.13069431177275792No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18560.13055362898290562No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18380.12928748387423522No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18230.12823236295034318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17720.12464495180911034No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG17500.12309744112073537No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17500.12309744112073537No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAGGCAGCTCCAACATTGGCAAGAATTATGTATCCTGGTACCAGCAG16690.11739978813171847No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTCCACAACACTCAGGC16310.11472681512452536No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16070.11303862164629813No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15790.11106906258836638No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15780.11099872119344026No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15760.11085803840358797No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15740.1107173556137357No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG15390.108255406791321No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15290.10755199284205966No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGACTTGGAGGC15000.10551209238920174No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACATAATTCTTGCCAATGTTGGA14880.10466799565008814No Hit
GGATAGCAGCCTGAGTGTTGTGGAATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14360.10101024311392914No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGATACATAATTCTTG14240.10016614637481554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)