FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421638
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43700.3073918958272078No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36210.254706191027533No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33220.2336741139446188No Hit
GCCTGGGATAGCAGCCTGAGTGTTGTGGAATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG31800.22368563586510773No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG29580.20806984619150584No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29340.20638165271327863No Hit
GTAATATACAGCCGTGTCCTCCTCTCTCAGGCTATTCATTTGCAAATACA28620.20131707227859694No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27290.19196166675342105No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25980.18274694401809743No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTCCAC25920.18232489564854062No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23590.1659353506307513No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC23390.1645285227322286No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATTCCACAACACTCAGG22890.16101145298592187No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22880.16094111159099575No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22730.15988599066710374No Hit
ATCCCAGGCAGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG21710.152711168384638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21300.14982717119266648No Hit
GATTATTACTGCGCTGCCTGGGATAGCAGCCTGAGTGTTGTGGAATTCGG21070.1482093191093654No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20550.1445515665732064No Hit
GAATTATGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACC20420.14363712843916665No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG20210.14215995914571783No Hit
GTATATTACTGTGTGAGATTGAGGGGTCCTGCAATGAGTACGATTTTCTT19520.13730640289581453No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19500.13716572010596229No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19360.1361809405769964No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG19210.13512581965310438No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGC19130.1345630884936953No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19070.1341410401241385No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18810.132312163856059No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18630.13104601874738858No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAGGCAGCTCCAACATTGGCAAGAATTATGTATCCTGGTACCAGCAG18060.1270365592365989No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18000.1266145108670421No Hit
GTCCAGGGTGGCTGACGTGCCAGACTTGGAGGCAGAGAATCGGTCAGGAA17820.1253483657583717No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17330.1219016374069911No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17170.12077617508817293No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17080.12014310253383773No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16890.11880661603024117No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15390.108255406791321No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15100.1062155063384631No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14800.10410526449067907No Hit
GGATAGCAGCCTGAGTGTTGTGGAATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14370.10108058450885528No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14330.10079921892915075No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT14300.10058819474437235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.042.4977531
GTATCAA25650.037.3166731
TATCAAC29000.032.7629662
TACGCTA552.675329E-432.4339160
TCAACGC30150.031.7380874
ATCAACG30700.031.1694893
CAACGCA31300.030.6840235
GTGGTAT3350.030.5042531
CGATTCG450.00475996730.4842234
AACGCAG31750.030.2533916
GGATGGG9450.030.07198170
TGGTATC3400.030.0482622
ATACGCT604.5214067E-429.65677359
CGCAGAG33900.028.4481538
AGAGTAC33500.028.29016511
CAGAGTA34850.027.09168610
TAACGGC907.128483E-626.72481236
GCAGAGT36000.026.413769
CATACGC700.001128451325.36043258
AATATAC9000.025.3509393
Produced by FastQC (version 0.11.5)