FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548380
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24470.4462234217148693No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGCCTGCATATGAGCAA21950.4002698858455815No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13380.24399139282978957No Hit
GTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAG13330.24307961632444655No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT12910.23542069367956525No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG12470.2273970604325468No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12270.22374995441117473No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11830.21572632116415624No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTT11410.20806739851927497No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11400.20788504321820636No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10900.19876727816477624No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10870.1982202122615704No Hit
GCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGG10690.1949378168423356No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10600.1932966191327182No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT10530.192020132025238No Hit
CTCCTGCACTGGAACCAGTGGTGATGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACATACAGGCAGAGC10480.19110835551989497No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10460.19074364491775775No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10210.1861847623910427No Hit
CAGTAATAGTCAGCCTCATCCTCAGCCTGGAGTCCAGAAATGGTCAGGGA10000.18235530106860207No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10000.18235530106860207No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9780.17834348444509282No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9660.1761552208322696No Hit
GACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGCCTGCATATGAGCAACAGTAATAGT9620.1754257996279952No Hit
GACTATTACTGTTGCTCATATGCAGGCGGGTATCGTTGGGTGTTCGGCGGAGGGACCGAGGTGACCGTCCTGGGT9580.17469637842372077No Hit
TTTCTGGACTCCAGGCTGAGGATGAGGCTGACTATTACTGTTGCTCATAT9570.17451402312265218No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTC9430.17196104890769176No Hit
CTATTACTGTTGCTCATATGCAGGCGGGTATCGTTGGGTGTTCGGCGGAG9400.17141398300448593No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGTCCAGAAATGGTCAGGGAGG9370.17086691710128013No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9100.16594332397242786No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9060.16521390276815348No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG8820.16083737554250702No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8810.16065502024143843No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8780.1601079543382326No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG8740.1593785331339582No Hit
CATACAGGCAGAGCCCCCAAACTCATGATTTTTCACGTCACTGCGCGGCCCTCAGGGGTCCCTGATCGGTTCT8590.15664320361792916No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8450.15409022940296874No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTTGGTGCTTATA8350.15226667639228272No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8310.1515372551880083No Hit
CCCCTGAGGGCCGCGCAGTGACGTGAAAAATCATGAGTTTGGGGGCTCTGCCTGTATGTTGTTGGTACCAGGAGA8000.14588424085488164No Hit
ACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGC7830.1427842007367154No Hit
CCTGAGGGCCGCGCAGTGACGTGAAAAATCATGAGTTTGGGGGCTCTGCC7740.14114300302709798No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG7730.1409606477260294No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC7590.13840767351106897No Hit
GTCCAGAAATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGACCCCTGAGGGCCGCG7170.13074875086618767No Hit
CCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATTTCTGGACTCCAGG6950.12673693424267843No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC6950.12673693424267843No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6810.12418396002771799No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT6790.12381924942558081No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGA6760.123272183522375No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6750.12308982822130639No Hit
GCTTATAACTATGTCTCCTGGTACCAACAACATACAGGCAGAGCCCCCAAACTCATGATTTTTCACGTCACTGCG6600.12035449870527737No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6580.11998978810314016No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6460.11780152449031693No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6380.11634268208176811No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA6360.1159779714796309No Hit
GTGGTGATGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACATACA6340.11561326087749371No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACAC6230.11360735256573909No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6210.11324264196360188No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC6170.11251322075932746No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6150.11214851015719028No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6070.11068966774864146No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC6000.10941318064116123No Hit
TAGCAGCCCCGTCGAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6000.10941318064116123No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5980.10904847003902404No Hit
CTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5960.10868375943688684No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5890.10740727232940661No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT5840.1064954958240636No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA5840.1064954958240636No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5760.1050366534155148No Hit
GCCTGTATGTTGTTGGTACCAGGAGACATAGTTATAAGCACCAACATCAC5750.10485429811444619No Hit
GGATGAGGCTGACTATTACTGTTGCTCATATGCAGGCGGGTATCGTTGGGTGTTCGGCGGAGGGACCGAGGTGAC5740.10467194281337758No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTTGGTG5690.10376016630803458No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAG5640.10284838980269156No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5540.10102483679200554No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC5530.10084248149093693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)