FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548380
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24810.45242350195120173No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGCCTGCATATGAGCAA20230.36890477406178196No Hit
GTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAG13790.25146796017360223No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13140.2396148656041431No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG12750.23250300886246764No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT12590.22958532404537No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11490.20952624092782376No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11130.2029614500893541No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTT10960.19986140997118787No Hit
CTCCTGCACTGGAACCAGTGGTGATGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACATACAGGCAGAGC10690.1949378168423356No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10500.19147306612203216No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC10450.19056128961668917No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10290.18764360479959152No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10280.1874612494985229No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10280.1874612494985229No Hit
GCCCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGG10180.1856376964878369No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10010.18253765636967065No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9940.18126116926219044No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT9700.176884642036544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9610.1752434443269266No Hit
CTATTACTGTTGCTCATATGCAGGCGGGTATCGTTGGGTGTTCGGCGGAG9440.17214340420876037No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9430.17196104890769176No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9370.17086691710128013No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG9340.17031985119807433No Hit
TTTCTGGACTCCAGGCTGAGGATGAGGCTGACTATTACTGTTGCTCATAT9070.1653962580692221No Hit
GACTATTACTGTTGCTCATATGCAGGCGGGTATCGTTGGGTGTTCGGCGGAGGGACCGAGGTGACCGTCCTGGGT9030.16466683686494765No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9020.16448448156387907No Hit
CAGTAATAGTCAGCCTCATCCTCAGCCTGGAGTCCAGAAATGGTCAGGGA8930.16284328385426164No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8790.16029030963930122No Hit
GTAATAGTCAGCCTCATCCTCAGCCTGGAGTCCAGAAATGGTCAGGGAGG8750.1595608884350268No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTC8720.159013822531821No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8610.15700791422006638No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG8500.15500200590831176No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8410.15336080819869433No Hit
GACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGCCTGCATATGAGCAACAGTAATAGT8060.14697837266129327No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG8010.14606659615595025No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC7940.14479010904847003No Hit
CATACAGGCAGAGCCCCCAAACTCATGATTTTTCACGTCACTGCGCGGCCCTCAGGGGTCCCTGATCGGTTCT7700.1404135818228236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7420.13530763339290275No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACAC7330.13366643568328532No Hit
CCCCTGAGGGCCGCGCAGTGACGTGAAAAATCATGAGTTTGGGGGCTCTGCCTGTATGTTGTTGGTACCAGGAGA7330.13366643568328532No Hit
GTGGTGATGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACATACA7310.1333017250811481No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTTGGTGCTTATA7290.1329370144790109No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG7180.13093110616725628No Hit
CCTGAGGGCCGCGCAGTGACGTGAAAAATCATGAGTTTGGGGGCTCTGCC7020.12801342135015864No Hit
GTCCAGAAATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAACCGATCAGGGACCCCTGAGGGCCGCG6700.1221780517159634No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6670.12163098581275758No Hit
ACCCAGGACGGTCACCTCGGTCCCTCCGCCGAACACCCAACGATACCCGC6660.12144863051168897No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6610.12053685400634598No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC6600.12035449870527737No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTCGA6580.11998978810314016No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6470.11798387979138553No Hit
CTTATGAGACACACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGA6470.11798387979138553No Hit
GCTTATAACTATGTCTCCTGGTACCAACAACATACAGGCAGAGCCCCCAAACTCATGATTTTTCACGTCACTGCG6420.11707210328604253No Hit
CCTCAGGGGTCCCTGATCGGTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATTTCTGGACTCCAGG6340.11561326087749371No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC6200.11306028666253327No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC6160.11233086545825888No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6100.11123673365184726No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC6050.11032495714650425No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT6050.11032495714650425No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6030.10996024654436705No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC5950.10850140413581823No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5920.10795433823261241No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC5850.10667785112513221No Hit
CTCCACTCCCGCCTCGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA5780.105401364017652No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5740.10467194281337758No Hit
GCCTGTATGTTGTTGGTACCAGGAGACATAGTTATAAGCACCAACATCAC5670.10339545570589737No Hit
GTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATTTCTGGAC5640.10284838980269156No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGTGGTGATGTTGGTG5540.10102483679200554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGA605.657054E-447.01952770
GTGGTAT2100.043.3466451
CGGGTAA405.7203757E-542.1502617
GGTATCA7000.040.4568671
GGATGGG2800.040.30245270
TGGTATC2450.037.14412
CGGGTTA400.002902954633.7202079
GTCTTAA400.002905552333.7140581
TCCGAGA2208.3127816E-1032.05877370
GTATCAA14650.031.9880831
AACGAAC450.00411956231.39428151
CCATTCG450.00441676930.95061145
ACGGGTA1750.030.82990516
GGTTACC553.7196334E-430.65753411
CCGGCGT450.00513270730.01459726
CTAGCCT450.00518335629.954394
AATGGTA2152.3530447E-829.5238970
ATAGCGG500.005721641229.35452863
AATAGCG500.00582229329.24997962
TGTGTCG5850.028.93509770
Produced by FastQC (version 0.11.5)