FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192423
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGGCA56260.4718124356876712No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45770.38384029828341115No Hit
CCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCT44120.370002926813723No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43160.3619520925040862No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39040.32740059525856174No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC38620.32387835524809566No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38510.32295586381678315No Hit
GGACAGGGGACTGGCCTGCCTTCTGCTGATACCAACAAACATACTTATCC37720.3163306980828112No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37680.315995246653243No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33330.27951490368770143No Hit
GACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCGCTGTCCCACGCCTGACAGTAATAGTCAG32020.2685288693693429No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31950.2679418293675986No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29540.24773088073611463No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGC28190.23640939498818792No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATC27820.23330646926468207No Hit
CCCCAGGACAGGCAGTCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT27750.23271942926293773No Hit
CTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAGCC27610.23154534925944903No Hit
GACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAG27570.23120989782988086No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCACCTGCTCTGG27210.22819083496376705No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGT27140.22760379496202268No Hit
GTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCTGAGCG27110.22735220638984655No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26320.22072704065587462No Hit
ACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCGCTGTCCCACGCCTGACAGTAATAGTCAGC26170.2194690977949939No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25580.21452118920886298No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25350.21259234348884581No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG23980.20110313202613503No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23970.20101926916874296No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23410.19632294915478818No Hit
GTATGTTTGTTGGTATCAGCAGAAGGCAGGCCAGTCCCCTGTCCTGGTCA23170.194310240577379No Hit
GAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATCCCT22510.1887752919895037No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCG22260.1866787205547025No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT21680.18181467482596358No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21430.17971810339116237No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCA20490.1718349947963097No Hit
GTAATACATGGCCGTGTCATCAGATCTCAGGCTGGTCAGGTCCATGTAGACTGTGCTCGTGGACGTGTCTTTGGA20370.1708286405076051No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20290.1701577376484687No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGG20160.16906752050237206No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19870.16663549763800262No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18920.1586685261857579No Hit
GTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC18730.15707513189530897No Hit
GTCTACATGGACCTGACCAGCCTGAGATCTGATGACACGGCCATGTATTACTGTGCGAGAGGCGATGGATACGC18520.15531401189007593No Hit
GACCAGGACAGGGGACTGGCCTGCCTTCTGCTGATACCAACAAACATACT18360.15397220617180313No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17960.15061769187612115No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGG17590.1475147661526153No Hit
ATGTATTACTGTGCGAGAGGCGATGGATACGCCTTTGGTATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT17300.14508274328824586No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGGCCTCCTATGAACTGACT17220.14441184042910946No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16950.1421475432795241No Hit
GGACAGGCAGTCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAGTA16700.1400509718447229No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16400.1375350861229614No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACAG16250.13627714326208065No Hit
GCAGAAGGCAGGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATT15930.13359353182553504No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15670.13141309753334177No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA15470.1297358403855008No Hit
AGGCAGGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCC15420.12931652609854052No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15400.12914880038375642No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14710.123362263223705No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14610.12252363464978451No Hit
CTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14520.12176886893325606No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14440.12109796607411966No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14210.11916912035410253No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGGCCTCCTATGAA14180.11891753178192638No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14120.11841435463757408No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14120.11841435463757408No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14110.11833049178018203No Hit
GAATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCAC14010.11749186320626154No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGT13960.1170725489193013No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13890.11648550891755695No Hit
GCACAGAATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCC13800.1157307432010285No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT13690.11480825176971596No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13640.11438893748275569No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13620.1142212117679716No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13530.11346644605144315No Hit
GTATTACTGTGCGAGAGGCGATGGATACGCCTTTGGTATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC13170.11044738318532936No Hit
GCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC12810.10742832031921559No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12570.10541561174180639No Hit
GCATCACCTGCTCTGGAGATAAATTGGGGGATAAGTATGTTTGTTGGTATCAGCAGAAGGCAGGCCAGTCCC12490.10474470888266998No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12490.10474470888266998No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12390.1039060803087495No Hit
CAGTAATACATGGCCGTGTCATCAGATCTCAGGCTGGTCAGGTCCATGTA12380.10382221745135745No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12330.10340290316439721No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12180.10214496030351645No Hit
GTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCACCTGCTCTGGAGATAA12120.10164178315916417No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACAGCCACT12120.10164178315916417No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12110.1015579203017721No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12010.1007192917278516No Hit
GTCCAAAGACACGTCCACGAGCACAGTCTACATGGACCTGACCAGCCTGAGATCTGATGACACGGCCATGTATTA12000.10063542887045956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)