FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192423
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGGCA48430.4061478183496964No Hit
CCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCT44390.3722672239633083No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43610.36572592108672847No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41750.3501274296118072No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38980.32689741811420947No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37160.3116343780688564No Hit
GGACAGGGGACTGGCCTGCCTTCTGCTGATACCAACAAACATACTTATCC36550.3065187437679414No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36340.3047576237627084No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36070.30249332661312306No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35760.2998935780339695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33560.2814437494077186No Hit
GACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCGCTGTCCCACGCCTGACAGTAATAGTCAG32840.275405623675491No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32710.27431540652939435No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGC28730.2409379892873586No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27440.2301196806837842No Hit
GTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCTGAGCG27090.22718448067506244No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGT26180.21955296065238591No Hit
CCCCAGGACAGGCAGTCAGCATCACCTGCTCTGGAGATAAATTGGGGGAT25910.2172886635028006No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25910.2172886635028006No Hit
GACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAG25860.21686934921584036No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25750.2159468577845278No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATC25680.21535981778278346No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCACCTGCTCTGG25650.21510822921060732No Hit
CTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAGCC25370.21276006920362991No Hit
ACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCGCTGTCCCACGCCTGACAGTAATAGTCAGC24780.207812160617499No Hit
GTATGTTTGTTGGTATCAGCAGAAGGCAGGCCAGTCCCCTGTCCTGGTCA22790.19112345199648112No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG22610.18961392056342424No Hit
GAGTGGCTGTGTTCCCAGAATTGGAGCCAGAGAATCGCTCAGGGATCCCT22520.18885915484689578No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22350.1874334862712309No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21870.18340806911641255No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCGGTTCCGAAGACCGCAGTGCCG21500.1803051433929067No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21460.17996969196333854No Hit
GTAATACATGGCCGTGTCATCAGATCTCAGGCTGGTCAGGTCCATGTAGACTGTGCTCGTGGACGTGTCTTTGGA20770.17418315480328708No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20650.1731768005145825No Hit
CAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGT20600.17275748622762224No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19880.16671936049539468No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19500.1635325719144968No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGG19180.1608489604779512No Hit
GTCCCACGCCTGACAGTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGC18760.1573267204674851No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18650.15640422903617257No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCA18540.15548173760486003No Hit
GACCAGGACAGGGGACTGGCCTGCCTTCTGCTGATACCAACAAACATACT18480.15497856046050773No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18280.15330130331266673No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18000.15095314330568935No Hit
GGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGG17990.15086928044829728No Hit
GTCTACATGGACCTGACCAGCCTGAGATCTGATGACACGGCCATGTATTACTGTGCGAGAGGCGATGGATACGC17220.14441184042910946No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA16670.13979938327254673No Hit
GGACAGGCAGTCAGCATCACCTGCTCTGGAGATAAATTGGGGGATAAGTA16660.1397155204151547No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16230.13610941754729655No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGGCCTCCTATGAACTGACT16200.1358578289751204No Hit
ATGTATTACTGTGCGAGAGGCGATGGATACGCCTTTGGTATTGACTACTGGGGCCAGGGAACCCTGGTCACCGT15430.1294003889559326No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15190.12738768037852338No Hit
GGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCGGCACTGCGGTCTTCGGAACCGGGACCAAGGTCACCGT15170.1272199546637393No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14950.1253749718011142No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14920.12512338322893804No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14830.12436861751240962No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACAG14750.12369771465327321No Hit
GCAGAAGGCAGGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCCTCAGGGATCCCTGAGCGATT14680.12311067465152886No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14620.12260749750717657No Hit
CTTCGGAACCGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14510.12168500607586401No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14370.12051092607237532No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14080.11807890320800589No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGAATCCGTGGCCTCCTATGAA13910.11665323463234104No Hit
GAATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCAC13750.11531142891406826No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13680.11472438891232391No Hit
AGGCAGGCCAGTCCCCTGTCCTGGTCATCTATCATGATAACAAGCGGCCC13530.11346644605144315No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13520.1133825831940511No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13480.11304713176448292No Hit
GCACAGAATCCGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCC13280.11136987461664193No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12930.10843467460792018No Hit
GTTCCGAAGACCGCAGTGCCGCTGTCCCACGCCTGACAGTAATAGTCAGC12820.10751218317660763No Hit
GCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC12800.10734445746182354No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12720.10667355460268714No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12690.10642196603051098No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12590.10558333745659049No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12560.10533174888441434No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12420.10415766888092563No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12310.1032351774496131No Hit
GTATTACTGTGCGAGAGGCGATGGATACGCCTTTGGTATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC12230.1025642745904767No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12230.1025642745904767No Hit
GTGTCCGTGTCCCCAGGACAGGCAGTCAGCATCACCTGCTCTGGAGATAA12170.1020610974461244No Hit
CAGTAATACATGGCCGTGTCATCAGATCTCAGGCTGGTCAGGTCCATGTA12100.10147405744438005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.038.760081
AATGGTA6000.036.88854670
CTCGCTA1100.036.82545523
TATCAAC27200.033.3395462
ATCAACG27750.032.918973
TCAACGC28050.032.5668954
TCGCTAT1255.456968E-1232.41184624
CAACGCA28700.031.7158575
GAGTTTA3850.031.5302491
AACGCAG29150.031.3458426
AGTTTAT3700.030.9780082
GTGGTAT2950.030.8622341
CGCAGAG30300.030.1675368
AGAGTAC30300.029.9524411
CAGAGTA30600.029.87679110
ACGCAGA30600.029.8655077
ACGCAGG1403.7851947E-529.6425870
TTATTCA3800.029.2768885
TAGGCAA2402.6120688E-928.81917470
TAGTCAC1202.8430804E-928.59649538
Produced by FastQC (version 0.11.5)