FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220305
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30900.2532153846784206No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26170.21445458307554258No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22180.1817578392287174No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGACCCGC21730.17807023653922585No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20920.17143255169814103No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20410.1672532686500506No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG19890.16299203887552702No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAACACCTCCAACTTTGAGAAAAATTATGTGTTCTGGTACCAGCAT18230.1493888822876248No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17800.14586517305099955No Hit
CCCAGGAACAACCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG17670.14479986560736866No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17510.14348871798443832No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17510.14348871798443832No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17440.1429150908994063No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17250.14135810309717653No Hit
GTCCAGGGTGGCTGACGTGCCAGAGTTGGAGCCAGAGAATCGGTCAGGAA16850.1380802340398507No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG16710.13693297986978667No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGACCCGCCGCCGAAAAGCACGGCACTCAGGCTGCCATCCCATGTT16690.13676908641692037No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16560.13570377897328947No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16480.1350482051618243No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16470.13496625843539115No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16420.1345565248032254No Hit
CCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCT16390.13431068462392598No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16240.13308148372742878No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16230.13299953700099565No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16180.13258980336882992No Hit
GGTCCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15800.1294758277643704No Hit
GATTATTACTGCGGAACATGGGATGGCAGCCTGAGTGCCGTGCTTTTCGGCGGCGGGTCCAAGCTGACCGTCCTA15780.1293119343115041No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15780.1293119343115041No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15410.1262799054334777No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15310.12546043816914623No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15060.1234117700083176No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15020.12308398310258502No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14400.11800328606372996No Hit
GACGTAGGACGGTCAGCTTGGACCCGCCGCCGAAAAGCACGGCACTCAGG14310.11726576552583165No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14150.11595461790290132No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14100.1155448842707356No Hit
GTTTGGGGGTTGTTCCTGGGAGATGCTGGTACCAGAACACATAATTTTTC14090.11546293754430244No Hit
CTTAAGAGGTGTCCAATGTCAGGTGCAGCTGGTGGAGTCTGGGGGGGGCG13800.11308648247774122No Hit
CCCTAAGACTCTCCTGTGCAGCGTCTGGATTCACCTTCTTTACCCATGGC13620.1116114414019446No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13540.11095586759047943No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13330.10923498633538335No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13210.10825162561818563No Hit
GTCTGGAGTCCGGTGATGTCCAGGGTGGCTGACGTGCCAGAGTTGGAGCC13200.10816967889175247No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12880.1055473836458918No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12870.10546543691945864No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12740.10440012947582776No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG12650.10366260893792945No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12540.10276119494716486No Hit
CAATAATATATGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTCCAGATA12480.10226951458856598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2600.043.73773640
CGTATGC2650.043.05573741
ATGCCGT2800.042.31477444
TCTCGTA2450.042.00844238
AGGGGGG3600.041.5543470
ACGAGAC2950.039.79220222
GTGGTAT4350.039.3088651
ATCTCCG3100.038.940710
GTATGCC3150.038.54049742
TCTCCGA3150.038.32259411
CCTAACG350.001551057738.3178832
CACGAGA3100.037.86211821
ATCTCGT2550.037.58050537
TCCGAGC3150.037.253513
GCCGTCT3200.037.20127546
GGTATCA11800.035.8012431
GTATCAA27950.034.907661
TGGTATC5000.034.8692782
CCCACGA3350.034.0285719
TCTACAT3100.033.8919832
Produced by FastQC (version 0.11.5)