FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220305
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30460.24960972871536213No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28130.23051614145643914No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25970.21281564854687968No Hit
GTCCTGGGCCCAGTCTGTCTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG21910.17954527761502248No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20100.16471292013062308No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18990.1556168334965439No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18610.15250285789208437No Hit
GTCTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC18010.14758605430609562No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGACCCGCCGCCGAAAAGCACGGCACTCAGGCTGCCATCCCATGTT17970.14725826740036302No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17700.1450457057866681No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGACCCGC17560.14389845161660403No Hit
ACCCTGGACATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTG17090.1400469554742462No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16830.1379163405869844No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAACACCTCCAACTTTGAGAAAAATTATGTGTTCTGGTACCAGCAT16760.13734271350195237No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16760.13734271350195237No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16570.1357857256997226No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16300.13357316408602768No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15650.1282466268678732No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15620.12800078668857376No Hit
GGTCCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG15460.12668963906564343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15440.12652574561277713No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15100.12373955691405017No Hit
GATTATTACTGCGGAACATGGGATGGCAGCCTGAGTGCCGTGCTTTTCGGCGGCGGGTCCAAGCTGACCGTCCTA15100.12373955691405017No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15000.12292008964971872No Hit
CCCAGGAACAACCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG14620.11980611404525916No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14540.11915054023379401No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14120.11570877772360189No Hit
CCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGATTCCT13940.11423373664780526No Hit
GTCCAGGGTGGCTGACGTGCCAGAGTTGGAGCCAGAGAATCGGTCAGGAA13820.11325037593060751No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13770.11284064229844179No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13730.1125128553927092No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13720.11243090866627604No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13470.11038224050544741No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13470.11038224050544741No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13290.10890719942965078No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAATGGAAGCCATGGGTAA13180.10800578543888618No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12950.10612101073092382No Hit
GACGTAGGACGGTCAGCTTGGACCCGCCGCCGAAAAGCACGGCACTCAGG12710.10415428929652833No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12670.10382650239079574No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12630.10349871548506318No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGTCCAGGGTGG12520.10259730149429855No Hit
GTTTGGGGGTTGTTCCTGGGAGATGCTGGTACCAGAACACATAATTTTTC12360.10128615387136823No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12360.10128615387136823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)