FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673823
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17710.2628286656881703No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT7820.11605421601815313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA200.00912416549.00797715
TCGTATG3200.044.2135740
CGTATGC3300.043.0865341
GCCGTCT3450.040.08372546
ATGCCGT3600.040.05444344
CTCGTAT3200.039.66686239
CGAGACA3500.039.24715823
TATACAC3750.039.2063833
GGTATCA15200.037.6157721
CACGATA350.001760649337.33941316
CCCACGA3800.036.97641819
GACGCTA400.001917068536.6970952
TTATACA4000.035.9391862
ATCTCGT3500.035.85888337
GGCAATC3550.035.80994433
ACGAGAC3850.035.6659922
CGTCTTC4050.035.37708348
GTATCAA35100.035.0921331
AGGGGGG4900.034.4773570
ATCTCCG3950.033.9101810
Produced by FastQC (version 0.11.5)