FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225154
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAATGC43650.3562817409076736No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31920.2605386751379827No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31530.25735540185152234No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC29370.2397249651880498No Hit
CTCCTGTACAACCTCTGGATTCACCTTCGCTCCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG29270.23890874126844464No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26930.21980910154968275No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26710.21801340892655127No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26400.21548311477577511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25240.2060149173083547No Hit
GTATAATACCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCATGCGAACTGTGGCTGCACCAT25130.20511707099678897No Hit
GTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCC24920.20340300076561804No Hit
GTATTATACACCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC24580.20062783943896031No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23700.19344506894643448No Hit
GACTTACACAGTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCATTGT23490.19173099871526356No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23380.19083315240369783No Hit
CTCCAGAGACAATGCCAAGAACTCATTGTCTCTGCAGATGAACAGCCTGA22480.18348713712725093No Hit
GATCAGCACTGAGCCCCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG21630.17654923381060666No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21500.1754881427151199No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20040.16357127348888384No Hit
GCACTGAGCCCCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC19840.16193882564967343No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19560.15965339867477885No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19420.15851068518733155No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18990.15500092233302915No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18880.15410307602146342No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18690.15255225057421354No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18680.152470628182253No Hit
GCGTAGACTGTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCCTGGAGCCTGGCGGACC18230.14879762054402956No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18070.14749166227266125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17960.1465938159610955No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC17170.14014564699621435No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16940.13826833198112237No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16710.1363910169660304No Hit
CTCCTGCAGGGCCAGTCAGATTGTTAGCAGCAGTCACTTAGCCTGGTACC16700.13630939457406988No Hit
GTCTGGGGGAGGCCTGGCCAAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAACCTCTGGATTCACCTTCGCTCC16490.13459532434289895No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16350.13345261085545165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16150.13182016301624122No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC16120.13157529584035965No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15870.12953473604134663No Hit
GTCTATTACTGTCAGGTGTATAATACCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCATGCGAA15710.12822877776997832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15690.12806553298605725No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT15370.1254536164433206No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15350.12529037165939955No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15280.1247190149156759No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG15060.12292332229254445No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14890.1215357416292156No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTGTTAGCAG14770.12055627292568935No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14720.12014816096588674No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC14640.11949518183020257No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14600.11916869226236049No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGCCAAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAAC14500.11835246834275528No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG14500.11835246834275528No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14380.11737299963922904No Hit
CTGCGTAGACTGTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCCTGGAGCCTGGCGGA14320.1168832652874659No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14220.1160670413678607No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13890.1133735024331635No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13770.11239403372963726No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT13730.11206754416179517No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13420.10953725001101904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13420.10953725001101904No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13420.10953725001101904No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT13200.10774155738788756No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG13200.10774155738788756No Hit
CCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAATA13010.10619073194063768No Hit
CCCTGAGACTCTCCTGTACAACCTCTGGATTCACCTTCGCTCCCTATGCC13000.10610910954867714No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12980.1059458647647561No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGACTGTGT12980.1059458647647561No Hit
GTGCAGCCACAGTTCGCATGATTTCCACCTTGGTCCCTTGGCCGAACGTC12970.10586424237279557No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12960.10578261998083506No Hit
CTATTACTGTCAGGTGTATAATACCTCACCTCCGACGTTCGGCCAAGGGA12810.10455828410142726No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12590.10276259147829578No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12520.10219123473457215No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG12410.1012933884230064No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12390.10113014363908536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)