FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225154
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAATGC41730.34061024165125364No Hit
CTCCTGTACAACCTCTGGATTCACCTTCGCTCCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG31210.25474348530878566No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29870.24380608478607588No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC28800.23507248884630014No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25360.20699438601188094No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25180.20552518295659156No Hit
GTATTATACACCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC25040.2043824694691443No Hit
GTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCC24920.20340300076561804No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24740.20193379771032866No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24690.20152568575052604No Hit
GTATAATACCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCATGCGAACTGTGGCTGCACCAT24460.19964837073543407No Hit
GACTTACACAGTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCATTGT23290.19009855087605312No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21710.17720221294629082No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21300.1738556948759095No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21210.17312109334826478No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20450.1669177915592652No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19890.16234693760947605No Hit
GATCAGCACTGAGCCCCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG19770.1613674689059498No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19530.1594085314988973No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG19420.15851068518733155No Hit
CTCCAGAGACAATGCCAAGAACTCATTGTCTCTGCAGATGAACAGCCTGA19270.15728634930792373No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18940.15459281037322653No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18620.15198089383048988No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18520.15116466991088467No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG18510.15108304751892415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18000.1469203055289376No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17810.1453694800816877No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17120.13973753503641176No Hit
GTCTGGGGGAGGCCTGGCCAAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAACCTCTGGATTCACCTTCGCTCC17120.13973753503641176No Hit
GCACTGAGCCCCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC16930.13818670958916185No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16310.13312612128760953No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16200.13222827497604384No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16180.1320650301921228No Hit
GTCTATTACTGTCAGGTGTATAATACCTCACCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCATGCGAA16110.13149367344839916No Hit
CTCCTGCAGGGCCAGTCAGATTGTTAGCAGCAGTCACTTAGCCTGGTACC15810.1290450016895835No Hit
GCGTAGACTGTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCCTGGAGCCTGGCGGACC15760.1286368897297809No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15560.1270044418905705No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15270.12463739252371539No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15210.12414765817195227No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14940.1219438535890182No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14860.12129087445333403No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGCCAAGCCGGGGGGGTCCCTGAGACTCTCCTGTACAAC14800.1208011401015709No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14780.12063789531764986No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14770.12055627292568935No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14750.12039302814176829No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14310.11680164289550538No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14140.11541406223217653No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTGTTAGCAG14110.11516919505629496No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13950.11386323678492663No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG13920.11361836960904508No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC13870.11321025764924246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13660.11149618741807153No Hit
CCCTGAGACTCTCCTGTACAACCTCTGGATTCACCTTCGCTCCCTATGCC13660.11149618741807153No Hit
CTGCGTAGACTGTGTAAGTCCCACTGCTACTGATGGATGCGACGTATTCCAGCCCCTTCCCTGGAGCCTGGCGGA13650.11141456502611101No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13620.11116969785022945No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13500.11019022914670318No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13480.11002698436278216No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT13460.1098637395788611No Hit
CCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAATA13420.10953725001101904No Hit
GTGCAGCCACAGTTCGCATGATTTCCACCTTGGTCCCTTGGCCGAACGTC13360.10904751565925591No Hit
GTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGACTGTGT13300.10855778130749279No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGG13250.10814966934769017No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12930.1055377528049535No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12630.10308908104613788No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12470.10178312277476953No Hit
CTATTACTGTCAGGTGTATAATACCTCACCTCCGACGTTCGGCCAAGGGA12270.10015067493555911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACG400.007137357352.1487970
TATTCGT200.00787440550.8716772
GGTATCA6500.046.4480861
GTATCAA19700.041.6716081
TATCAAC20800.039.1320572
CGAGTGA750.001820941637.0835870
ATCAACG22350.036.4167143
CAACGCA22650.036.082635
AACGCAG22750.036.07756
TCAACGC22650.035.9343764
CAGATGA18000.033.99328270
AATGGTA5350.033.7911170
CGCAGAG25100.032.7117468
AGAGTAC25200.032.1872311
GTGCTAG951.0370059E-832.137351
ATACGTA851.3959288E-731.94164711
CGAAATT553.050836E-431.71546748
CCGAAAT553.0817717E-431.66096347
GTAGTTT1103.1118805E-431.60532670
GCAGAGT26200.031.2126779
Produced by FastQC (version 0.11.5)