FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592543
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA55310.34730616378961193No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40940.2570731214165018No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA40410.25374511080705514No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39950.2508566487686675No Hit
ATATTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT39570.24847052795434724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38330.24068423898130223No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT33070.20765530349886943No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31830.19986901452582442No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29930.18793841045422321No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28990.18203590107143106No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27670.17374727087431863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27530.17286817373220062No Hit
CTCCTAAGGTTCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT26160.1642655802700461No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25730.1615654961906837No Hit
GTATAAGCCCTGTGGTACAGTAATACAGGGCTTCGTCCTCGGTTTTCAGGCTGTCCATTTGCAGAAACAGCGTG25550.16043522843653202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24170.15176984232136903No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24120.1514558790563269No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA23420.14706039334573698No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23190.14561616232654315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23150.14536499171450945No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22380.14052995743286054No Hit
TTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22150.13908572641366668No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21870.13732753212943072No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA21540.13525537458015263No Hit
GTCCCTGACCGACTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC20870.131048266828588No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20720.1301063770334616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20160.12658998846498964No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA19770.12414107499766097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19760.12407828234465255No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19620.12319918520253456No Hit
AACCAGGACAGCCTCCTAAGGTTCTCATTTACTGGGCATCTACCCGGGAA18940.11892928479796148No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA18730.11761063908478453No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAA17750.11145695908995863No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17740.11139416643695021No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17450.10957317949970581No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17410.10932200888767211No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17210.10806615582750355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17080.107249851338394No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16980.10662192480830972No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG16370.10279157297479566No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16360.10272878032178721No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT16250.10203806113869451No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15950.10015428154844169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA200.00820480450.34611527
GTGGTAT3500.048.7940181
GTCGTAT507.335075E-847.96796839
CGTATGC8500.046.99877541
TCGTATG8550.046.20773340
GCCGTCT8700.045.3102246
GGTATCA14350.044.570341
CCGTCTT8850.044.3060447
ATCTCGT7400.044.1878737
TATGCCG9300.043.1817743
ACGAGAC9250.042.7375222
CGAGACT9100.042.7137323
CGTCTTC9500.042.16943448
CCCACGA9300.042.13427719
GATCTCG7750.042.1062236
CTTGAAA9700.041.88232457
TATACAC9800.041.345073
TGGTATC4050.041.340852
ATGCCGT9650.040.99522844
ATCTCCG9700.040.7343810
Produced by FastQC (version 0.11.5)