FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1592543
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84070.5278978338418492No Hit
CTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA44880.2818134267018222No Hit
ATATTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT39270.24658674836409442No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37780.23723064306583871No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC31630.19861316146565586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31210.1959758700393019No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAAGACT30800.1934013712659564No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA30680.19264785942985527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30660.1925222741238384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26530.1665889084313579No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26280.1650190921061472No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA26050.16357486108695338No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24640.15472109701276512No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24640.15472109701276512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24010.1507651598732342No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23860.14982327007810778No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22910.1438579680423072No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA22770.14297887090018918No Hit
CTCCTAAGGTTCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT22000.13814383661854027No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21340.13399952151998407No Hit
TTATAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT21210.13318321703087452No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20640.12960403580939414No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20560.12910169458532672No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19840.12458062356871998No Hit
GTATAAGCCCTGTGGTACAGTAATACAGGGCTTCGTCCTCGGTTTTCAGGCTGTCCATTTGCAGAAACAGCGTG19520.12257125867245029No Hit
GTCCCTGACCGACTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18780.11792460234982666No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18300.11491055500542215No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGAACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA18240.11453379908737157No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18220.11440821378135473No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG18210.11434542112834631No Hit
GTCCCAGACCCGCTGCCACTGAGTCGGTCAGGGACCCCGGATTCCCGGGT17970.11283839745614405No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17780.11164533704898393No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17770.1115825443959755No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17500.10988714276474795No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAGTCGGTCAGGGACCCCG17200.10800336317449513No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17150.107689399909453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16800.10549165705415803No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATA15990.1004054521604754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG200.005870902454.7928959
CCGAGCG400.006060820554.3510170
CTAACGC352.7351496E-547.7676053
GTGGTAT4450.046.638091
GGTATCA15150.040.434081
TGGTATC5150.040.2736132
GTATCAA34250.034.402591
TTCGATC601.4051024E-534.2724539
ATCAACG40450.028.932173
TCAACGC40650.028.7057654
CGATAGT500.006624459328.49083552
CAACGCA41650.027.9362745
CCTAACG606.494241E-427.8775672
ATAGGCA1306.498043E-427.87231370
TAGGCAT1050.007723754727.60686570
TATCAAC42650.027.4527032
AACGCAG42550.027.4239566
TAAGCCC7250.026.7481784
CGCAGAG44150.026.2786468
ACGCAGA44300.026.1896677
Produced by FastQC (version 0.11.5)