FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075972
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52340.4864438851568629No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA46350.43077329149829174No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC44900.417297104385616No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40780.3790061451413234No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39930.37110631131665134No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37590.34935853349343665No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAATA34020.31617923142981413No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC32750.30437595030353953No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32740.3042830110820728No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30990.28801864732539506No Hit
GGTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAACAATCGG29720.2762153661991204No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG29090.27036019524671645No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAAT28210.26218154375764424No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATA27660.2570698865769741No Hit
TATGACAACAGTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGT26920.25019238418843615No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGT26290.24433721323603216No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTC25740.23922555605536203No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25190.2341138988746919No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC25100.23327744588149132No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24330.22612112582855318No Hit
GTTGTCATATGACTGGCAATAATAATAAGCCTCATCGTCAGCCTGGAGCC24280.2256564297212195No Hit
GGCCATCACTGGGCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGT23960.22268237463428414No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23910.2222176785269505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23270.2162695683530798No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21840.20297925968333747No Hit
TTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGC21470.19954050848906849No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21450.19935463004613505No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20980.19498648663719872No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19760.18364790161825773No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAACATCATAACCTGCCCCGATATTGGAG19270.17909387976638796No Hit
CATCATAACCTGCCCCGATATTGGAGCTGCTCCCAGTGCAGGAAATGGTGACCGTCTGCCCTGGGGCCCCAGACA18680.17361046569985092No Hit
TCACTGGGCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGTCATAT17780.16524593576784527No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17530.16292245523117702No Hit
GCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGACAACTTCCA17210.15994840014424166No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGGGAGCAG17180.15966958247984148No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17030.15827549415784053No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16790.15604495284263903No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGC16710.15530143907090518No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16650.1547438037421048No Hit
GGCTTATTATTATTGCCAGTCATATGACAACAGTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGTCAC16610.1543720468562379No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16580.1540932291918377No Hit
TAATAAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAAT16310.15158387021223602No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAACATCATAACCT15880.14758748368916663No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15340.14256876572996324No Hit
GTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA15330.1424758265084965No Hit
GTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAACAATCGGC15180.14108173818649555No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15120.14052410285769518No Hit
CTCCTCATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATT15070.14005940675036155No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14930.13875825764982733No Hit
CCCCTGAGGGCCGATTGTTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAA14900.13847943998542714No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT14890.1383865007639604No Hit
GTGCAGGAAATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG14690.1365277163346258No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14570.13541244567702504No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14400.13383247891209066No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC14360.13346072202622372No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14350.133367782804757No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14330.13318190436182353No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14300.13290308669742334No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCC14100.13104430226808877No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGTCTATG14100.13104430226808877No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14030.13039372771782165No Hit
GCCCCCAAACTCCTCATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGT13950.1296502139460878No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13400.12453855676541768No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13360.12416679987955077No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13290.12351622532928365No Hit
ATATTGGAGCTGCTCCCAGTGCAGGAAATGGTGACCGTCTGCCCTGGGGC13270.12333034688635021No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13240.12305152922195No Hit
GGGCAGACGGTCACCATTTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGA13180.12249389389314964No Hit
GTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGGGAGCAGCT12820.11914808192034737No Hit
GTTCCTGGAAGTTGTCGGTACCAGTGAACATCATAACCTGCCCCGATATT12620.11728929749101277No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG12470.11589520916901183No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12410.11533757384021145No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT12350.11477993851141109No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12340.11468699928994434No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAAT12290.1142223031826107No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGG12270.11403642473967723No Hit
ATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGG12230.11366466785381031No Hit
CTGCACAGGGTCCGGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC12230.11366466785381031No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12210.11347878941087686No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG12180.11319997174647667No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12070.11217764031034264No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11860.11022591665954132No Hit
GGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG11840.11004003821660786No Hit
GCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGTCATATGACAACA11740.10911064600194056No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11670.10846007145167347No Hit
GTCATATGACAACAGTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCA11670.10846007145167347No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11530.10715892235113925No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11430.10622953013647196No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11390.10585777325060504No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG11370.10567189480767157No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT11270.10474250259300429No Hit
CATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTC11190.10399898882127044No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC11140.1035342927139368No Hit
CCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGTC11060.10279077894220295No Hit
GTCAGGGACCCCTGAGGGCCGATTGTTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGTCGGTA11020.10241902205633605No Hit
GTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT11020.10241902205633605No Hit
GTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTAGG10910.10139669062020201No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAATACACTGTTGTCATATGACTGGCAAT10830.10065317684846817No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10810.10046729840553473No Hit
CTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCT10800.10037435918406798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTG100.008992897133.91470
AGGGGGG2800.057.3917270
CGTATGC2550.052.77910641
TCGTATG2500.052.3577240
CAACGCT200.00725108351.9434843
TCTCGTA2350.051.12854838
CCCACGA2700.048.97815319
GTATGCC2850.048.5191842
ACGAGAC2750.048.0831722
ATCTCCG2800.047.22454510
AATCTCG2550.046.97699736
CTTATAC3150.046.2848931
CGAGACA2950.045.97688323
CCGTCTT3000.045.3491747
GCCGTCT3000.045.27556646
GTCTAAC306.8747345E-445.20851
ATCTCGT2700.044.4338737
ATGCCGT3100.043.63384644
TCTCCGA3150.041.9773711
GCAATCT2850.041.9317334
Produced by FastQC (version 0.11.5)