FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075972
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48280.44871056124137054No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44030.4092113921180105No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC41030.3813296256779916No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41030.3813296256779916No Hit
GTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGGGAGCAG40440.3758462116114546No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37580.3492655942719699No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35740.33216477752209167No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34480.3204544356172837No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33730.313483994007279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29060.27008137758231626No Hit
GGTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAACAATCGG28950.26905904614618226No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27920.2594863063351091No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGT26780.24889123508790192No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAATA25420.23625150096842668No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25400.23606562252549323No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25370.23578680486109305No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATA25010.23244099288829076No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24570.22835166714375468No Hit
GGCCATCACTGGGCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGT24260.22547055127828605No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTC22750.21143672883680986No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22590.20994970129334223No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22300.20725446387080704No Hit
CTGCACAGGGTCCGGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC22230.20660388932053994No Hit
TATGACAACAGTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGT22030.20474510489120532No Hit
GTTGTCATATGACTGGCAATAATAATAAGCCTCATCGTCAGCCTGGAGCC20920.19442885130839838No Hit
TTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGC20760.1929418237649307No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20680.19219830999319684No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC20470.19024658634239552No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAAT19940.18532080760465885No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19480.18104560341718928No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19360.17993033275958856No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19260.17900094054492124No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18700.1737963441427844No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18340.1704505321699821No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17680.16431654355317798No Hit
GTCCGGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTG17160.15948370403690804No Hit
GTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGGGAGCAG17150.15939076481544132No Hit
TCACTGGGCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGTCATAT17050.158461372600774No Hit
GTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA17040.15836843337930728No Hit
GGGTCCGGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTC17020.15818255493637381No Hit
CTCCTCATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATT16700.15520849984943846No Hit
GCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGACAACTTCCA16690.15511556062797172No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGC16360.15204856631956964No Hit
ATGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCAC16310.15158387021223602No Hit
GGCTTATTATTATTGCCAGTCATATGACAACAGTGTATTTGTCTTTTATGTCTTCGGAACTGGGACCAGGGTCAC16040.1490745112326343No Hit
GCACAGGGTCCGGGGCCCAGTCTATGCTGACGCAGCCGCCCTCAGTGTCT15440.14349815794463053No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15090.140245285193295No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14970.13913001453569424No Hit
GCCCCCAAACTCCTCATCTATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGT14830.13782886543516004No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14710.13671359477755926No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14710.13671359477755926No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGTCTATG14690.1365277163346258No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14620.1358771417843587No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC14620.1358771417843587No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14540.13513362801262488No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14520.1349477495696914No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAACATCATAACCTGCCCCGATATTGGAG14300.13290308669742334No Hit
CATCATAACCTGCCCCGATATTGGAGCTGCTCCCAGTGCAGGAAATGGTGACCGTCTGCCCTGGGGCCCCAGACA14250.1324383905900897No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14060.13067254538222184No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14030.13039372771782165No Hit
GGGCAGACGGTCACCATTTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAGGTTATGATGTTCACTGGTACCGA13980.12992903161048802No Hit
ATATTGGAGCTGCTCCCAGTGCAGGAAATGGTGACCGTCTGCCCTGGGGC13950.1296502139460878No Hit
GTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGGGAGCAGCT13770.12797730795968668No Hit
GTTATGATGTTCACTGGTACCGACAACTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAACAATCGGC13660.12695497652355267No Hit
GTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAACATCATAACCT13540.1258397058659519No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13500.12546794898008498No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13440.1249103136512846No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13320.12379504299368385No Hit
GTGCAGGAAATGGTGACCGTCTGCCCTGGGGCCCCAGACACTGAGGGCGG13300.1236091645507504No Hit
GGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG13290.12351622532928365No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13110.12184331934288253No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13050.12128568401408216No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT13050.12128568401408216No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAAAAGACAAATACACTGTTGTCATATGACTGGCAAT13030.12109980557114869No Hit
TAATAAGCCTCATCGTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAAT12740.11840456814861354No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12630.1173822367124795No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG12510.11626696605487877No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12470.11589520916901183No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12340.11468699928994434No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12180.11319997174647667No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12080.11227057953180938No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA12040.11189882264594246No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGACGGTCACCATTTCCTGCACTGG12020.111712944203009No Hit
ATGCTAACAACAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGG11640.10818125378727328No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACGTGGAGCC11570.10753067923700618No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11550.10734480079407271No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11440.10632246935793868No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11420.10613659091500523No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11360.10557895558620484No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCGGGGCCCAGTCTATG11310.1051142594788712No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11140.1035342927139368No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG10990.10214020439193584No Hit
GCTCCAGGCTGACGATGAGGCTTATTATTATTGCCAGTCATATGACAACA10820.10056023762700145No Hit
CCCCTGAGGGCCGATTGTTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGTTGTCGGTACCAGTGAA10760.10000260229820106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)