FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344562
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65190.4848419039062535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55310.4113607256489474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49320.3668109019889005No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG42580.31668305366357224No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42270.31437747013525597No Hit
GGTCTAGTCAGAGCCTCCTGTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC41640.3096919294164196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40590.3018826948850257No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39090.2907266455544631No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA36650.2725794719767478No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36420.27086887774606155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35670.2652908530807802No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34600.25733287122497883No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGTAGAG33170.24669743752984247No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32870.24446622766372988No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG32130.23896257666065232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31430.23375642030638977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31310.23286393635994473No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31110.2313764631158697No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30650.22795527465449714No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30470.22661654873482964No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG30240.22490595450414338No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGGAGTTT29800.22163351336717832No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA29380.21850981955462076No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT29310.2179892039191945No Hit
AGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCC29140.21672485166173072No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA28260.21017996938780065No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27960.20794875952168812No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGATGTGGAGACTGCCCTGGCTTCTGCAGGTA27630.20549442866896434No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26900.20006515132809047No Hit
GTGTAGGGAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTC26770.1990982937194417No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26130.19433837933840167No Hit
GGCCAAAAGTGTAGGGAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCA26020.1935202690541604No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25750.1915121801746591No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTAGAAACT25520.1898015859439728No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG25340.18846286002430532No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC25200.1874216287534528No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25070.18645477114480402No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA24700.18370294564326523No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24540.18251296704800524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24170.17976114154646644No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG23550.17514997448983385No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23460.1744806115300001No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC23200.1725468963127026No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC23100.17180315969066506No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22640.16838197122929252No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22490.16726636629623626No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21860.16258082557739992No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA21770.16191146261756617No Hit
GTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCC21750.16176271529315867No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT21740.1616883416309549No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTAGTAAAC20450.15209413920667103No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19980.14859857708309474No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19960.1484498297586872No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19870.14778046679885345No Hit
CTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG19580.14562363059494468No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCCCTACACTTTTGGCCAGGGGA19160.14249993678238712No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18960.14101246353831212No Hit
AGTCAGAGCCTCCTGTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAT18890.14049184790288585No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG18870.14034310057847835No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18780.13967373761864457No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18670.13885562733440332No Hit
GCCACTGAACCTGTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGATGTGGAGACTGCCCTGG18500.13759127507693955No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18470.1373681540903283No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18310.13617817549506828No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18030.13409571295336326No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17850.13275698703369573No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT17690.13156700843843572No Hit
GTAATACACGGCCGCGTCTGCGGCGGTCACTGTGCCCAGCTTCAGGGAGA17510.1302282825187682No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17480.13000516153215694No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17370.12918705124791569No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17350.12903830392350818No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17160.1276252043416369No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16980.12628647842196938No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16950.12606335743535813No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16790.12487337884009811No Hit
GATGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTAGTAAACAGGAGGCTCTGA16770.1247246315156906No Hit
CTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16740.12450151052907936No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA16730.1244271368668756No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16520.12286528996059683No Hit
GCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT16450.12234467432517059No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16420.12212155333855931No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16380.12182405868974432No Hit
GTAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTA16250.12085720108109556No Hit
CTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCT16220.1206340800944843No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA16190.12041095910787306No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16140.12003909079685429No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGC16090.11966722248583554No Hit
CTCTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16060.11944410149922427No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16040.11929535417481679No Hit
GAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT15970.11877473853939052No Hit
ATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT15730.1169897706465005No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC15700.11676664965988924No Hit
GTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC15680.11661790233548175No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15420.11468418711818422No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15280.11364295584733171No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15260.11349420852292419No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15170.11282484556309044No Hit
CCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA14950.11118862499460791No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14940.11111425133240416No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14550.10821367850645787No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14550.10821367850645787No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGT14330.10657745793797534No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGTTTACTAATGGATACAACTATTTGG13970.1039000060986403No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13930.1036025114498253No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13870.1031562694766028No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13760.10233815919236153No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13600.1011481805971015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT5450.049.89848770
TACGTAT650.001006496641.83796370
GGTATCA10500.040.5720251
CGTATGC2050.040.4121441
TCGTATG1800.040.21226540
ATCTCGT1900.039.70367437
CACGAGA2750.039.3645321
CTAGTGA5650.037.84438369
CGAGACA2800.037.4743323
ACGAGAC2800.037.46038422
ATGCCGT2250.037.00119444
CTTATAC2850.036.7758141
GTATGCC2350.036.7750942
TCTCCGA3050.036.5799711
GGGCGTA656.38669E-736.410794
GTATCAA27600.036.3825231
ACAAAGG6800.034.9931570
CCCACGA3100.034.9084619
ATCGCGT400.002675562434.2895437
TCCGAGC3200.033.80624813
Produced by FastQC (version 0.11.5)