FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344562
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61690.4588111221349406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46820.3482174864379627No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45740.34018513091995756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41400.3079069615235296No Hit
GGTCTAGTCAGAGCCTCCTGTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC39980.2973459014905969No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37810.28120681679238296No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA37270.27719063903338037No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG37160.27637252874913915No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36580.2720588563413216No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33550.249523636693585No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33440.2487055264093437No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30990.2304839791694247No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30070.2236416022466796No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29890.2223028763270121No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29450.21903043519004703No Hit
GTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAAAATCAGTAGAG29450.21903043519004703No Hit
GTTTATTACTGCATGCAAGCTCTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA29070.2162042360263045No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28950.21531175207985948No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28800.2141961471468032No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT27370.2035607134516668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27300.20304009781624052No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27030.20103200893673923No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTAGGGAGTTT26890.19999077766588674No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG26460.19679271019112546No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25980.19322277440534538No Hit
AGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCC25800.19188404848567786No Hit
GATTTTACACTGAAAATCAGTAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTAGAAACT25330.18838848636210156No Hit
GTGTAGGGAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTC25240.1877191234022678No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25030.18615727649598904No Hit
GTCCCTGACAGGTTCAGTGGCAGTGCATCAGGCACAGATTTTACACTGAA24990.18585978184717403No Hit
GTGTAAAATCTGTGCCTGATGCACTGCCACTGAACCTGTCAGGGACCCCG24560.18266171437241271No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24510.18228984606139398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24480.18206672507478272No Hit
GTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGATGTGGAGACTGCCCTGGCTTCTGCAGGTA24390.18139736211494897No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24180.1798355152086702No Hit
GGCCAAAAGTGTAGGGAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCA24140.17953802055985518No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23890.1776786790047614No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23870.17752993168035391No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC22860.17001819179777503No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22660.1685307185537No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC22070.1641426724836787No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA22070.1641426724836787No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21890.16280394656401118No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21690.16131647331993615No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21470.15968025275145364No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAATCTGTGCCTGATGCACTGCCA20910.15551532766804357No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20810.15477159104600605No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20240.15053229230039225No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT20100.1494910610295397No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19470.14480552031070343No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA19320.14368991537764716No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19250.1431692997422209No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19030.14153307917373836No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19020.1414587055115346No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18920.14071496888949708No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18910.14064059522729336No Hit
GTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCC18780.13967373761864457No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18490.1375169014147358No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTAGTAAAC18400.13684753845490205No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18220.13550881253523453No Hit
TTATTACTGCATGCAAGCTCTAGAAACTCCCTACACTTTTGGCCAGGGGA17990.13379821830454824No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17980.1337238446423445No Hit
AGTCAGAGCCTCCTGTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAT17960.13357509731793699No Hit
CTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG17810.13245949238488072No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG17480.13000516153215694No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17360.12911267758571193No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16740.12450151052907936No Hit
GCCACTGAACCTGTCAGGGACCCCGGAGGCCCGACTAGAACCCAAATAGATCAGGAGATGTGGAGACTGCCCTGG16700.12420401588026436No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16650.1238321475692456No Hit
GAGTTTCTAGAGCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAAAAT16540.12301403728500433No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16500.12271654263618934No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16480.12256779531178183No Hit
CTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16370.12174968502754056No Hit
GTAATACACGGCCGCGTCTGCGGCGGTCACTGTGCCCAGCTTCAGGGAGA16320.12137781671652181No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15900.11825412290396425No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15730.1169897706465005No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGT15660.11646915501107424No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15640.11632040768666672No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15600.11602291303785173No Hit
GTTTACTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC15560.11572541838903673No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15540.11557667106462921No Hit
CTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCT15360.1142379451449617No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15310.11386607683394295No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT15260.11349420852292419No Hit
GATGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTAGTAAACAGGAGGCTCTGA14970.11133737231901542No Hit
CTCTAGAAACTCCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14660.10903178879069914No Hit
GCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTT14620.10873429414188411No Hit
GTAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGCTCTA14580.10843679949306911No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14520.1079905575198466No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGC14500.10784181019543912No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14470.10761868920882785No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14300.10635433695136409No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC14240.10590809497814159No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA13960.10382563243643654No Hit
ATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT13810.10271002750338028No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13610.10122255425930526No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13600.1011481805971015No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13560.1008506859482865No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG13540.100701938623879No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13450.10003257566404523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT200.00799088450.6833219
TAGTGAT5650.042.7720570
GTGGTAT3900.042.4542771
TGGTATC3900.042.4289932
GGTATCA12300.040.108621
GTATCAA29250.035.4652021
TATCAAC32500.032.0035822
ATCAACG33500.031.233593
TCAACGC33650.031.1888644
CAACGCA34400.030.6058375
TAAGACG450.00515137429.9954625
ACAAAGG6900.029.87297870
AACGCAG35250.029.7709856
CTAGTGA5850.029.34166169
CGCAGAG36800.028.6087388
ACGCAGA36800.028.5170447
TCATACC500.006751744528.379754
AGAGTAC37000.028.17945111
CAGAGTA37500.027.71477710
GCAGAGT38250.027.3488049
Produced by FastQC (version 0.11.5)