FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190584
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38280.3215228828877257No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCA35280.29632516479307636No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34940.29346942340901605No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33240.2791907164887148No Hit
GGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACT33110.27809881537127995No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCCGAATATTACTGCAGTTCCTAC32940.27667094467924985No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC32400.27213535542221295No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31660.26591991829219946No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31350.2633161540890857No Hit
GTATTCGGCCCTCAGGGGTTTCTGGTCGCTTCTCTGGCTCCAAGTCTGGC29850.25071729504176105No Hit
GACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCA29670.24920543195608205No Hit
ACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCAT28250.23727851205794806No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG27700.2326589304072623No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27140.2279553563629278No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCATAATTTCTGTAGGAA26220.2202280561472353No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25260.2121647863569475No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23460.19704615550015792No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22940.19267855103041867No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG22910.19242657384947218No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20640.17336030049118753No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20300.17050455910712725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20270.17025258192618076No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20210.1697486275642878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19240.16160136538035114No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATATAGCGGTGATGTT18720.15723376091061192No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGC18620.15639383697412362No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18370.15429402713290286No Hit
TAGTTATAGGTACCAACATCACCGCTATATCCAGTGCAGGAGATGGTGAT18240.15320212601546804No Hit
GCTATATCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACGCAGACACGGAGGCAGGCTGAGTCAGGTCAGA17840.1498424302695148No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC17580.1476586280346452No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17530.147238666066401No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17400.14614676494896622No Hit
CTCCTGCACTGGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACTACACCCAGGCAAAGC16620.1395953582443574No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16600.13942737345705972No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16470.13833547233962493No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16240.13640364728570178No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16200.13606767771110648No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16190.13598368531745764No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16120.13539573856191583No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15470.1299362329747418No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15260.12817239270811637No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15050.1264085524414909No Hit
GTACCAACTACACCCAGGCAAAGCCCCCAAACTCATTCTTTATGAGGTCA15030.12624056765419323No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14810.12439273499391895No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14520.12195695557810284No Hit
GTCGATCACCATCTCCTGCACTGGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACTACA14230.11952117616228675No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC14220.1194371837686379No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14100.11842927504485193No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13930.11700140435282182No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13710.11515357169254752No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13650.11464961733065454No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13500.11338973142592207No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13340.11204585312754077No Hit
GCCGAATACTGACCTCATAAAGAATGAGTTTGGGGGCTTTGCCTGGGTGT13330.11196186073389193No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13290.11162589115929662No Hit
CTATAACTATGTCTCGTGGTACCAACTACACCCAGGCAAAGCCCCCAAAC13250.11128992158470129No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13020.10935809653077816No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC13010.10927410413712935No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12980.10902212695618285No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12460.10465452248644362No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12390.1040665757309018No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12370.10389859094360415No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12110.10171478870873453No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12100.1016307963150857No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12080.10146281152778804No Hit
ACCTATAACTATGTCTCGTGGTACCAACTACACCCAGGCAAAGCCCCCAAACTCATTCTTTATGAGGTCAGTATT12070.10137881913413922No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11950.10037091041035325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA252.889312E-453.89470324
TCGTATG1500.048.2573940
CCGTCTT1550.045.19228447
GCCGTCT1400.044.92092546
CTAGTAC307.1337505E-444.8707543
CGTATGC1700.044.69804841
AGGGGGG2350.044.1823670
GGTATCA9200.043.893461
TCTCGTA1650.043.65065838
CGAGACG2000.042.0804723
CGCTCAT2050.041.1960428
ATCTCGT1750.041.0526637
CCCACGA2050.041.0437719
ATGCCGT1750.039.76844844
GTATGCC2100.039.57766342
ATCGCGG350.001522330638.4638816
ACGCTCA2200.038.35003327
ATCTCCG2250.037.39229610
TATACAC2350.037.2331773
GACGCTC2350.035.86741626
Produced by FastQC (version 0.11.5)