FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190584
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACT40230.33790139964924776No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36520.30674022160553144No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCA34210.2873379786726514No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33970.2853221612250795No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33820.284062275320347No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCCGAATATTACTGCAGTTCCTAC32290.27121143909207585No Hit
GTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG31840.26743178137787843No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30510.25626079302258387No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30230.2539090060004166No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29030.24382991876255689No Hit
GTATTCGGCCCTCAGGGGTTTCTGGTCGCTTCTCTGGCTCCAAGTCTGGC27810.23358284673739943No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26130.21947212460439583No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCATAATTTCTGTAGGAA26060.218884177848854No Hit
ACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCAT26000.218380223486961No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25000.2099809841220779No Hit
GACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGCCA22380.18797497698608412No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGCCTGCCTCCGTGTCTGCGTCTCCTGGACAGTCGATCAC21960.18444729645283325No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21740.18259946379255892No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG21410.17982771480214751No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21350.17932376044025453No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGATATAGCGGTGATGTT21300.17890379847201038No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21190.1779798821418732No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20840.17504014836416415No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20840.17504014836416415No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19300.16210531974224412No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19300.16210531974224412No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19120.1605934566565652No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCACTTCCGAAGACAAAAGCACTGC18930.1589976011772374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18330.15395805755830752No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG18130.1522782096853309No Hit
CTCCTGCACTGGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACTACACCCAGGCAAAGC17690.14858254436478233No Hit
TAGTTATAGGTACCAACATCACCGCTATATCCAGTGCAGGAGATGGTGAT17020.14295505399031064No Hit
GCTATATCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACGCAGACACGGAGGCAGGCTGAGTCAGGTCAGA16620.1395953582443574No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16440.13808349515867843No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16300.1369076016475948No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGACCTGACTCAGC15990.13430383744448102No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15650.13144809606042077No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15520.13035619494298595No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15470.1299362329747418No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15230.12792041552716987No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14840.12464471217486545No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14670.12321684148283532No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14650.12304885669553765No Hit
GTCGATCACCATCTCCTGCACTGGATATAGCGGTGATGTTGGTACCTATAACTATGTCTCGTGGTACCAACTACA14400.12094904685431689No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14370.12069706967337039No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14260.11977315334323324No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14210.11935319137498906No Hit
GTACCAACTACACCCAGGCAAAGCCCCCAAACTCATTCTTTATGAGGTCA14160.11893322940674492No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13600.11422965536241036No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13490.11330573903227323No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13450.11296976945767792No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13130.11028201286091531No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13060.1096940661053735No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12930.1086021649879387No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12850.10793022583874803No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12840.10784623344509922No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12750.10709030190225972No Hit
CTATAACTATGTCTCGTGGTACCAACTACACCCAGGCAAAGCCCCCAAAC12420.1043185529118483No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12370.10389859094360415No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC12270.10305866700711583No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12200.10247072025157403No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12190.10238672785792519No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12150.10205075828332986No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12110.10171478870873453No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12070.10137881913413922No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12050.10121083434684154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG200.00810735950.4977524
TACGGAC200.00815058150.4298673
CTGTACG306.278378E-446.0491141
TACGTAT700.00117569840.53597370
GGTATCA7900.040.0135231
TACGAAA350.001296699539.73990644
GTACGAA350.001309770639.6589243
CGTAAGC350.001485704938.6549630
GTATCAA20600.037.0565261
AATGGTA5150.034.435970
GTATACA502.167143E-433.61854
TATCAAC23100.032.7494162
GTGGTAT2900.031.3091891
TGTACGA450.00454164230.7777742
ACGGGAC553.7711847E-430.58925616
TATACAG553.7916744E-430.560995
ATCAACG25000.030.39243
ACGTAAG450.005117414530.03577429
TCAACGC25350.029.9715214
ATAGCAC450.00524544229.8843673
Produced by FastQC (version 0.11.5)