FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730325
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGAACCTCACCTCGAACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT59140.3417855027234768No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57960.3349659746001474No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51140.29555141375175187No Hit
GTTCCATACTGCTGACAGTAATAAACTGCAAAATCCTCAGGTTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTC46740.2701226648173031No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40410.23353994191842573No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39460.2280496438530334No Hit
GTCTGGGACAGACTTCACTCTCACCGTCAGCAGACTGGAACCTGAGGATT37610.21735801077832198No Hit
GTATATTATTGTGCGAGAAGTCCTTTCGGGGTTGGTGGCACATTCGACCC37510.21678008466617543No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36920.2133703206045107No Hit
CATCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA36540.2111742013783538No Hit
ATAATATACAGCCGTGTCTGCGGCGGTCACAGACTCCATCTTCAGCGAGA35120.20296765058587257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35060.20262089491858465No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC34840.20134945747186223No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC34700.20054036091485702No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34170.19747735252048024No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32920.19025327611864823No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT32530.18799936428127662No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG32490.18776819383641802No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32480.18771040122520335No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGTCAACCTCTCCTGCAGGGCCAGTCAGCCTATTAGCGG32270.18649675638969557No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC32010.18499414849811452No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC30830.1781746203747851No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30290.17505381936919365No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29000.16759857252250301No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG28960.16736740207764436No Hit
GTCTCCGGTGGCTCCGTCACCAGTGGTGATTATTACTGGGGCTGGATCCG28840.1666738907430685No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT28830.16661609813185385No Hit
CACCTGCACTGTCTCCGGTGGCTCCGTCACCAGTGGTGATTATTACTGGG28590.16522907546270207No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT28440.16436218629448224No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG28360.163899845404765No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGACAGAGTCAACCTCTCCTGC28060.16216606706832531No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC27930.16141476312253478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27870.16106800745524685No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27780.16054787395431494No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTT27630.1596809847860951No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27590.15944981434123645No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT26640.15395951627584414No Hit
CCATAGATGAGGACCCTGGGAGGCTGGCCGAGTTTCTGTTGGTACCAGCCTAAGTAGTTGCCGCTAATAGGCTGA25960.1500296187132475No Hit
GCCTATTAGCGGCAACTACTTAGGCTGGTACCAACAGAAACTCGGCCAGC25540.14760232904223194No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25350.14650426942915348No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25280.1460997211506509No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA25050.14477049109271378No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG24710.14280554231141548No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24690.14268995708898619No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC24510.14164969008712236No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24040.13893343736003352No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA23550.13610159941051536No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC23270.134483406296505No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG23010.13298079840492394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22740.1314203979021282No Hit
CTCCTGCAGGGCCAGTCAGCCTATTAGCGGCAACTACTTAGGCTGGTACC22250.12858855995261004No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC21880.12645023333766778No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21660.12517879589094535No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG21480.12413852888908154No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG21440.1239073584442229No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21340.12332943233207634No Hit
GACTTCACTCTCACCGTCAGCAGACTGGAACCTGAGGATTTTGCAGTTTATTACTGTCAGCAGTATGGAACCTCA21330.12327163972086169No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21070.12176903182928062No Hit
TCGCATGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGA20910.12084435004984613No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC20810.12026642393769957No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC20700.11963070521433836No Hit
GAGTCAACCTCTCCTGCAGGGCCAGTCAGCCTATTAGCGGCAACTACTTA20520.11859043821247453No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20480.11835926776761592No Hit
GTTTATTACTGTCAGCAGTATGGAACCTCACCTCGAACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA20350.11760796382182538No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT20270.11714562293210812No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT20250.11703003770967882No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19670.11367806625922877No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19420.11223325097886236No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19390.11205987314521837No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC19180.11084622830971061No Hit
GTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCACAGAGGTCACGCTCAGGTGGTC18780.10853452386112436No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA18730.10824556080505107No Hit
TCTATGGTGCATCCAACAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT18730.10824556080505107No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18730.10824556080505107No Hit
ATATTATTGTGCGAGAAGTCCTTTCGGGGTTGGTGGCACATTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGT18570.10732087902561659No Hit
GTCTTTGTCTCCAGGGGACAGAGTCAACCTCTCCTGCAGGGCCAGTCAGC18520.1070319159695433No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC18400.10633840463496742No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18160.10495138196581566No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18030.10420007802002514No Hit
GTAATAAACTGCAAAATCCTCAGGTTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17750.10258188490601476No Hit
GCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGG17500.10113706962564836No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA17400.1005591435135018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)