FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997689
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCC36560.3664468586904336No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34640.3472023847110673No Hit
CCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC30820.30891390002295305No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29580.29648517724461226No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAG28840.2890680362317315No Hit
GTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAG27640.27704023999462757No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27540.27603792364153557No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAAT22050.221010755856785No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21720.21770311189158145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21710.21760288025627225No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20280.20326975640705672No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19340.19384798268799194No Hit
GATTATTACTGCTGCTCACATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT18950.18993894891093316No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18770.18813477947536755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18290.183323660980526No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18150.1819204180861972No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18110.1815194915449604No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16760.16798822077821846No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG16570.16608381970734368No Hit
TCTCTGGGCTCCAGGCTGAGGATGAAGCTGATTATTACTGCTGCTCACAT16530.16568289316610688No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16260.1629766390127585No Hit
GTAATAATCAGCTTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16230.1626759441068309No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15980.1601701532241009No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15770.1580652888826077No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15660.15696274089420653No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15640.15676227762358813No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15300.15335440202307532No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15260.15295347548183852No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCAC15080.1511493060462729No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14840.14874374679885216No Hit
GTCAGTCACCATCTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCA14620.14653865082204975No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTG14550.14583702937488535No Hit
CATACAGGCACCTCCCCTGTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14290.14323100685684617No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14100.1413266057859714No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA14010.1404245210681886No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC13140.13170436879628822No Hit
CCCCTGAGGGCCGCTTAGTGACATCATACATCTTGAGTTTGGGGGCTTTGCCGGGGTGCTGTTGGTACCAGGAGA13080.13110297898443302No Hit
GTTCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG13040.13070205244319624No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12930.12959950445479504No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12910.12939904118417664No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12800.12829649319577544No Hit
CTCCTGCACTGGAATCAGCACTGATGTTGATATTCATACTTATGTCTCCTGGTACCAACAGCACCCCGGCAAAGC12550.12579070231304545No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12420.12448769105402585No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12040.12067888891227627No Hit
GCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCA12030.12057865727696708No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12030.12057865727696708No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12020.12047842564165787No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12020.12047842564165787No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCT11510.11536661224088869No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11320.1134622111700139No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCCGGACAGTCAGTCACCATCTCCTGCACTGGAATCAGCA11270.11296105299346791No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11200.1122594315463035No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTGGGAGCCAGAGAAG11010.11035503047542872No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11000.11025479884011953No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10950.10975364066357352No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10750.10774900795738952No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10620.10644599669836993No Hit
GCCTGTATGTGAGCAGCAGTAATAATCAGCTTCATCCTCAGCCTGGAGCC10520.10544368034527793No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10510.10534344870996874No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10470.10494252216873194No Hit
GAACAGAACAGGGGAGGTGCCTGTATGTGAGCAGCAGTAATAATCAGCTT10380.10404043745094915No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10340.10363951090971235No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10240.10263719455662036No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10190.10213603638007435No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCCAGTCTGGCAACACGG10060.10083302512105476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)