FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111399
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69050.621289024013878No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAATATCGAAATGCCAGTCTCCATGTATAGAACA62020.5580354130244853No Hit
GTTCTATACATGGAGACTGGCATTTCGATATTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGG48080.43260791129018467No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39080.35162889295383565No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCAC38860.34964940583894716No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37830.3403818070737872No Hit
GTATAGAACACAGTAATAGTCACACTCATCATCTGCCTGGGCCCCCGTGA35620.3204969592378615No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT31210.28081724025305044No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30930.2782978930159196No Hit
CCCCAGGCCTGGCTCCACGCAGGCTCATATATAACACAAACACTCGCTCT30100.2708298279915674No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTC29350.2640815764635383No Hit
GGCTCATATATAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAG29280.2634517396542556No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29050.26138227585232665No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG28780.2589529053022362No Hit
CTACTAATTCCTTCACCAGCTGGTACCAGCAGACCCCAGGCCTGGCTCCACGCAGGCTCATATATAACACAAACA27090.24374684519241066No Hit
CAGTAATAGTCACACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC26260.23627878016805848No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25230.22701118140289853No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24640.2217025568675156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24560.2209827433711925No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24370.21927318631742515No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCGATTCTCAGACTGTGGTGACCCAGGAGCCAT24110.21693379245437505No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGTGAAGGAATTAGTAGAGATCGAA23680.21306479491163838No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23340.21000558755226523No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCG23290.20955570411706326No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22610.20343728939831687No Hit
CTATTACTGTGTTCTATACATGGAGACTGGCATTTCGATATTCGGCGGAG22480.20226759246679185No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAATATCGAAATGCCAGTCTCCATGTATAGAACACAGTAATAGT22330.20091794216118603No Hit
ATATATGAGCCTGCGTGGAGCCAGGCCTGGGGTCTGCTGGTACCAGCTGG22100.1988484783592571No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21850.19659906118324744No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21830.19641910780916666No Hit
GTAATAGTCACACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG21710.19533938756468197No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21690.19515943419060122No Hit
CTCATATATAACACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAG21660.19488950412948006No Hit
GACTATTACTGTGTTCTATACATGGAGACTGGCATTTCGATATTCGGCGGAGGGACCAAGTTGACCGTCCTGGGT21540.1938097838849954No Hit
ATATGAGCCTGCGTGGAGCCAGGCCTGGGGTCTGCTGGTACCAGCTGGTG21320.19183029677010685No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21230.19102050658674338No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21180.19057062315154144No Hit
CCCCAGAAGAGCGAGTGTTTGTGTTATATATGAGCCTGCGTGGAGCCAGG21170.19048064646450105No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAAGTCACTTTCAGGGAGA20670.18598181211248166No Hit
CCCCTGGAGGGACAGTCACACTCACCTGTGGCTTGAGTTCTGGTTCGATC19930.17932353727149294No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19760.17779393359180637No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19260.17329509923978698No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT18940.17041584525449457No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18940.17041584525449457No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAATATCGAAATGCCAGTCT18100.16285780354310198No Hit
ATACATGGAGACTGGCATTTCGATATTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAGGCTGCCCC17680.1590787826874057No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17390.15646945876323445No Hit
CACCTGTGGCTTGAGTTCTGGTTCGATCTCTACTAATTCCTTCACCAGCTGGTACCAGCAGACCCCAGGCCTGG17020.15314032134274008No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16950.1525104845334574No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16730.15053099741856885No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16560.14900139373888224No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAAC16090.14477248944798404No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16060.14450255938686288No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCGATT15940.14342283914237822No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15880.14288297902013586No Hit
CAGTCACACTCACCTGTGGCTTGAGTTCTGGTTCGATCTCTACTAATTCCTTCACCAGCTGGTACCAGCAGACCC15820.14234311889789356No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15550.13991374834780307No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15380.1383841446681165No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15280.1374843777977126No Hit
CTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAA14970.1346951004994606No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14950.13451514712537982No Hit
GTCACACTCACCTGTGGCTTGAGTTCTGGTTCGATCTCTACTAATTCCTT14670.13199579988824897No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATC14500.13046619620856237No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14270.12839673240663343No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14250.12821677903255266No Hit
CCTGTGGCTTGAGTTCTGGTTCGATCTCTACTAATTCCTTCACCAGCTGGTACCAGCAGACCCCAGGCCTGGCTC14090.12677715203990644No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14030.12623729191766414No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13930.12533752504726026No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13870.12479766492501793No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG13770.12389789805461406No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13740.12362796799349289No Hit
GTGTGACTATTACTGTGTTCTATACATGGAGACTGGCATTTCGATATTCGGCGGAGGGACCAAGTTGACCGTC13560.1220083876267659No Hit
GTGTTATATATGAGCCTGCGTGGAGCCAGGCCTGGGGTCTGCTGGTACCA13490.12137855081748318No Hit
GCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGAGTGTTTGTGTTATATATGAGC13360.12020885388595816No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAACTTGGTC13320.1198489471377966No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13290.11957901707667544No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCC13040.11732959990066573No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12990.1168797164654638No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12950.11651980971730226No Hit
GGCCCAGGCAGATGATGAGTGTGACTATTACTGTGTTCTATACATGGAGA12770.11490022935057526No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCGATTCTCAGA12750.11472027597649449No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12700.11427039254129255No Hit
GAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAGTGTG12600.11337062567088868No Hit
GAATTAGTAGAGATCGAACCAGAACTCAAGCCACAGGTGAGTGTGACTGT12570.11310069560976752No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCGATTCTCAGACTGTGGTGACC12550.11292074223568672No Hit
GATGATGAGTGTGACTATTACTGTGTTCTATACATGGAGACTGGCATTTC12420.1117510453041617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12390.11148111524304054No Hit
GAGCCAGGCCTGGGGTCTGCTGGTACCAGCTGGTGAAGGAATTAGTAGAG12230.11004148825039432No Hit
ATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGA12150.10932167475407122No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11930.10734218763918268No Hit
TAGTCACACTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAG11900.10707225757806153No Hit
GGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCA11880.10689230420398074No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAGTGTGACTATTACTGTGTTC11860.10671235082989998No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11810.10626246739469804No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11730.10554265389837493No Hit
GTTCTGGTTCGATCTCTACTAATTCCTTCACCAGCTGGTACCAGCAGACC11690.10518274715021338No Hit
GTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAATCGACCCCTGATC11620.10455291034093066No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11600.1043729569668499No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC11470.10320326003532485No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11360.10221351647788059No Hit
GATCAGGGGTCGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA11210.10086386617227476No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11200.10077388948523437No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGGGTCGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT11120.10005407598891126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)