FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004687485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929769
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47200.5076529761693496No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA44740.48119479139442156No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG42270.4546290530228476No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGCCAAGATATGAGCTGCAATAATAAT40080.4310748153573629No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37930.4079507920784625No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37260.4007447011031773No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37130.39934650434677854No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35560.38246058967334895No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGC33200.3570779408648815No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31010.33352370319939684No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30930.33266327442622845No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGCCAAGATATGAGCTG28690.30857126877751356No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27920.3002896418357678No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26830.2885662998013485No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAG24460.263076097396235No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC24350.2618930078331284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24130.25952682870691535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22830.24554486114292906No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22470.24167293166367126No Hit
TCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT22370.24059739569721078No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21140.22736830330974683No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20200.21725826522501823No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC20170.21693560443508011No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATT19920.2142467645189289No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19540.21015972784637904No Hit
GTCTGAGGACGAGGCTGATTATTATTGCAGCTCATATCTTGGCAGCTACA19430.2089766382832725No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18820.20241586888786353No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18800.20220076169457143No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18660.20069501134152676No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17890.192413384399781No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17280.18585261500437206No Hit
GATTATTATTGCAGCTCATATCTTGGCAGCTACAATTTTGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGT16710.1797220599955473No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16490.1773558808693342No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15840.17036489708734104No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15720.16907425392758846No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15010.161437948565719No Hit
GCTCATATCTTGGCAGCTACAATTTTGTCTTCGGAAGTGGGACCAAGGTC14810.15928687663279803No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG14780.15896421584285989No Hit
GTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACA14690.15799623347304545No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14190.15261855364074303No Hit
TCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAGCGACTGGAAACC14010.15068258890111413No Hit
GACCATCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATATCTTGGCAGCTACAATTTTGT13920.14971460653129973No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13910.14960705293465365No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13890.14939194574136158No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13660.14691821301850244No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13550.1457351234553959No Hit
GTAATAAATGGCCGTATCGTCAGATGTCAGGCTCGCCACCTCCATGTAGACTGTAGTCGTGGATGTATCTGTGGT13420.14433692669899728No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC13310.14315383713589075No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13240.1424009619593684No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13190.14186319397613817No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13170.14164808678284607No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13020.14003478283315532No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12880.13852903248011064No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12830.1379912644968804No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12830.1379912644968804No Hit
GATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAG12800.13766860370694226No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12410.13347401343774637No Hit
ATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGG12220.13143049510147142No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGGGTTTCCAGTCGCTTCTCTGGCTCCAAGTCCGGCAACACGGCCTC11980.1288492087819663No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATTGGGGTTTCCAGTCGCTTCTCTGGCT11710.12594526167252296No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11690.12573015447923086No Hit
CCCCTGAGGGCCGAGAAGTGACCTCATAAATGATGACTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA11680.1256226008825848No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT11500.12368663614295593No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11480.12347152894966384No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11300.12153556421003496No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11280.12132045701674288No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11150.11992226026034424No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11140.11981470666369819No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11010.11841650990729954No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10940.11766363473077722No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC10930.11755608113413116No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10910.11734097394083906No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10840.11658809876431674No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10790.1160503307810865No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10570.11368415165487342No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10450.11239350849512084No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA10320.11099531173872221No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACATCTCCACCATGGCCTGGGCT10310.11088775814207615No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10310.11088775814207615No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG10170.10938200778903148No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC10130.10895179340244728No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10040.10798381103263284No Hit
CTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTC9930.10680072146952631No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG9840.10583273909971186No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9800.10540252471312767No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA9760.10497231032654349No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG9620.1034665599734988No Hit
CATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAGTCATCATTTATGAGGT9600.1032514527802067No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9480.1019608096204541No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG9480.1019608096204541No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9460.10174570242716201No Hit
CTTCTCGGCCCTCAGGGGTTTCCAGTCGCTTCTCTGGCTCCAAGTCCGGC9430.10142304163722386No Hit
GGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAGCGACTGGAAA9430.10142304163722386No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA9380.10088527365399362No Hit
GGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGT9350.10056261286405548No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9340.10045505926740944No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG9310.10013239847747128No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9310.10013239847747128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)