FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929769
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43100.46355600154446963No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG41640.4478531764341465No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40400.43451653045003646No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA39380.42354606359213953No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGCCAAGATATGAGCTGCAATAATAAT38960.4190288125330055No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35110.3776206778242768No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34890.3752544986980637No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33350.3586912448145722No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32780.35256068980574745No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30110.3238438795012525No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGC28470.30620508965130044No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27310.29372887244035883No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAGCTGCCAAGATATGAGCTG27190.29243822928060625No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACAAAATTGTAG23990.25802107835387067No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23960.2576984175639326No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23900.25705309598405623No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23890.2569455423874102No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22970.24705061149597374No Hit
TCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT21200.22801362488962312No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20800.2237114810237812No Hit
GTCTGAGGACGAGGCTGATTATTATTGCAGCTCATATCTTGGCAGCTACA20760.223281266637197No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20740.2230661594439049No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC20380.21919422996464713No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19710.2119881389893619No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19530.210052174249733No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19440.20908419187991856No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATT19060.2049971552073687No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT18900.20327629766103195No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18040.19402668834947173No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17520.18843390132387722No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17430.18746591895406278No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16750.18015227438213147No Hit
GATTATTATTGCAGCTCATATCTTGGCAGCTACAATTTTGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGT16140.17359150498672252No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16110.17326884419678437No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16040.17251596902026203No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15750.1693969147175266No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG15180.16326635970870185No Hit
GACCATCTCTGGGCTCCAGTCTGAGGACGAGGCTGATTATTATTGCAGCTCATATCTTGGCAGCTACAATTTTGT14250.15326387522061932No Hit
GCTCATATCTTGGCAGCTACAATTTTGTCTTCGGAAGTGGGACCAAGGTC14220.15294121443068118No Hit
GATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAG14140.1520807856575128No Hit
GTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACA14140.1520807856575128No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14110.15175812486757462No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13890.14939194574136158No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13830.14874662416148526No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT13810.14853151696819317No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13450.14465958748893543No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13230.14229340836272233No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG13190.14186319397613817No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT13100.1408952116063237No Hit
GTAATAAATGGCCGTATCGTCAGATGTCAGGCTCGCCACCTCCATGTAGACTGTAGTCGTGGATGTATCTGTGGT12960.13938946125327906No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12920.13895924686669484No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12780.13745349651365016No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12560.1350873173874371No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC12520.1346571030008529No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12480.1342268886142687No Hit
TCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCGGACTTGGAGCCAGAGAAGCGACTGGAAACC12290.1321833702779938No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12080.12992474474842677No Hit
ATAATAATCAGCCTCGTCCTCAGACTGGAGCCCAGAGATGGTCAGGGAGG12050.1296020839584886No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11920.12820388720208997No Hit
CTCCTGCACTGGAGCCAGCAGTGACATTGGGGTTTCCAGTCGCTTCTCTGGCTCCAAGTCCGGCAACACGGCCTC11890.12788122641215183No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11830.12723590483227554No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11770.12659058325239925No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC11720.126052815269169No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC11560.12433195772283223No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGACATTGGGGTTTCCAGTCGCTTCTCTGGCT11520.12390174333624802No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11220.12067513543686657No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG11180.1202449210502824No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC11150.11992226026034424No Hit
CCCCTGAGGGCCGAGAAGTGACCTCATAAATGATGACTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA10940.11766363473077722No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10930.11755608113413116No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10710.1151899020079181No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG10690.11497479481462601No Hit
GGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGT10650.1145445804280418No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10650.1145445804280418No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10620.11422191963810366No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC10480.11271616928505898No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC10220.10991977577226172No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10200.10970466857896963No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10150.10916690059573936No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10010.1076611502426947No Hit
GTATCAACGCAGAGTACGGGGCTGGGGTCTCAGGAGGCAGCGCTCTCAGGACATCTCCACCATGGCCTGGGCT9900.10647806067958815No Hit
CTTCTCGGCCCTCAGGGGTTTCCAGTCGCTTCTCTGGCTCCAAGTCCGGC9880.10626295348629607No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9870.10615539988965No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9820.10561763190641976No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9770.10507986392318952No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG9730.10464964953660534No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG9630.10357411357014486No Hit
CTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA9620.1034665599734988No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9610.10335900637685273No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCC9520.10239102400703831No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9520.10239102400703831No Hit
CATTGGTACTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAGTCATCATTTATGAGGT9480.1019608096204541No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG9450.10163814883051597No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA9410.10120793444393177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)