FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171189
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50040.4272581112015225No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGTAGGTGCCTGCATACGAG49450.42222049558184027No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37490.32010205013879056No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGTAG36530.3119052518423585No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCATCAGTGATGTTGGTGGTTATAACT34210.29209632262598095No Hit
GATTATTACTGCTGCTCGTATGCAGGCACCTACACTTTCGTGGTATTCGG33830.2888517566336432No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33640.28722947363747436No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32870.28065495833721116No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30720.2622975454858268No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28000.23907328364593589No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCGTAT27370.23369413476390233No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26750.2284003691974566No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26220.22387505347130138No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCATCAGTGATGTT24940.2129459890760586No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23760.20287075783669417No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23670.20210230799640366No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23420.19996772510670777No Hit
CTCCTGCACTGGAACCATCAGTGATGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAGGC23170.19783314221701195No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23060.1968939257455458No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22630.19322244317526888No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22390.19117324360116086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21930.1872456110841205No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21730.18553794477236382No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21500.1835741285138436No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG21350.1822933787800261No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21330.18212261214885045No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGAAAGTGTAGG21300.18186646220208694No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21170.1807564790994451No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20380.17401119716800617No Hit
GTGTAGGTGCCTGCATACGAGCAGCAGTAATAATCAGCCTCATCTTCAGC20310.17341351395889135No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC19960.17042509791331714No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19920.1700835646509658No Hit
GTCAGTCACCATCTCCTGCACTGGAACCATCAGTGATGTTGGTGGTTATAACTCTGTCTCCTGGTACCAACAGCA19830.16931511481067532No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19350.16521671566245927No Hit
GTTATAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAGGCCCCCAAA18720.15983756678042568No Hit
CACCTACACTTTCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT18490.15787375052190553No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18220.155568401001034No Hit
CCCCTGAGGGCCGCTTAGCGACATCATAAATTATGAGTTTGGGGGCCTTGCCTGGGTGCTGTTGGTACCAGGAGA18070.15428765126721647No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17670.15087231864370312No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17450.14899388570077077No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGCCAACACGG17230.1471154527578384No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17210.14694468612666273No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC17050.1455785530772574No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15730.13430795541966326No Hit
GAGTTATAACCACCAACATCACTGATGGTTCCAGTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACA15690.13396642215731192No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGGCAGACTTGGAGCCAGAGAAG15660.13371027221054843No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15500.1323441391611431No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC15320.13080723948056205No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGCCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG15280.13046570621821071No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15280.13046570621821071No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14640.12500117402058933No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14490.12372042428677181No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14360.12261044118412998No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14330.12235429123736646No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA14060.12004894171649494No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13880.11851204203591392No Hit
GGTTATAACTCTGTCTCCTGGTACCAACAGCACCCAGGCAAGGCCCCCAAACTCATAATTTATGATGTCGCTAAG13780.1176582088800356No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT13620.11629207583063023No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13510.11535285935916408No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCT13420.11458440951887357No Hit
GCTTGGTCCCTCCGCCGAATACCACGAAAGTGTAGGTGCCTGCATACGAGCAGCAGTAATAATCAGCCTCATCTT13290.1134744264162317No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13060.11151061015771152No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12920.11031524373948183No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12640.10792451090302249No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12600.10758297764067114No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12520.10689991111596847No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC12420.10604607796009012No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTC12400.10587531132891448No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12260.10467994491068477No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT12150.1037407284392186No Hit
GGCAAGGCCCCCAAACTCATAATTTATGATGTCGCTAAGCGGCCCTCAGG12010.10254536202098892No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11930.10186229549628625No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11780.10058154576246875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.043.7002261
TGGTATC2600.032.620362
CCAATAC751.9031995E-631.6634983
GTATCAA19500.031.6634961
TTAGATC450.004418443430.95123346
AATTACG450.004491871730.84714744
ACGAGAC1001.7020284E-830.54309322
CCCACGA1001.706212E-830.53526719
CCCGTGA604.25649E-429.96347661
CTTAACG500.006588142428.52229758
TATCAAC21950.028.1293052
ACGTTAA500.00727142327.95164948
TACGTTA500.007323074627.9111547
ATCAACG22550.027.380853
AATGGTA5150.027.17576470
TGCAACG500.00834340127.1749526
TCAACGC23100.026.7289284
GTGCTAG2050.026.4781861
CGACCGG2050.026.47705511
CACGAGA1307.52334E-926.10297821
Produced by FastQC (version 0.11.5)