FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471813
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACA68930.46833395275079104No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA64970.44142836080398806No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62760.4264128663084237No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACACAGTAATAAT62590.42525782827030334No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG59040.40113791629779055No Hit
GATTATTACTGTGTGATATTTGTGGGAAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT58570.39794457583945786No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG53220.36159484934567093No Hit
GTCTATTACTGTGCGAGACGGTCTCCTGAACCAGGATATAATGGCTTTAAGGGCCGAGCCTTTGATATCTGGGGC49850.33869791882528555No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAG45000.30574536303185257No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42190.2866532636958635No Hit
GTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGG42150.28638149003983526No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39150.26599846583771175No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC38290.260155332233103No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT37430.25431219862849425No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36590.24860495185189965No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36470.24778963088381473No Hit
GTAGATGAGCGTGCGTGGAGGCTGGCCTGGGGTCTGCTGGTACCAGCTGG34440.2339971178403778No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32980.2240773793953444No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG31570.21449735802034636No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAGCTGCCCTCACCATCACGGGGGCCCAGG31560.21442941460633924No Hit
CCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT31440.21361409363825432No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGGCTGG30200.2051891103013766No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTC30080.20437378933329167No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29980.2036943551932209No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29760.20219960008506516No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCC29290.1990062596267325No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28620.1944540508882582No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAACA28440.1932310694361308No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28430.19316312602212374No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28030.19044538946184061No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28000.19024155921981936No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGACTGTGGTGACCCAGGAGCCAT27870.18935829483772734No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27740.18847503045563535No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAG27660.1879314831435787No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGG27090.18405870854517523No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26770.18188451929694874No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26290.17862323542460898No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25280.17176095060989405No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24250.16476277896716499No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAGCTGC24130.16394745799908006No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23460.1593952492606058No Hit
CCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTC23010.15633779563028727No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATT22870.15538658783418818No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22270.1513099829937635No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22230.15103820933773515No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT21970.1492716805735511No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21660.1471654347393317No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21500.14607834011521845No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21410.14546684938915475No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG21210.14410798110901318No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG21110.1434285469689424No Hit
AGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTCCCTCCAGGGGACACTGAGAGCGATGGCTC21090.14329266014092823No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGA21010.1427491128288716No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21000.14268116941486453No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20680.140506980166638No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG20110.13663420556823455No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC19680.1337126387659302No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19560.13289731779784525No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19340.13140256268968953No Hit
CACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTACCCC19190.13038341147958335No Hit
CAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCC18940.12868482612940638No Hit
GTTTCTGGTGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGATTCG18930.1286168827153993No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18850.12807333540334268No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18820.12786950516132145No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18730.12725801443525775No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18590.12630680663915864No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTAGACACGTCC18490.12562737249908784No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18100.1229775793528118No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17890.12155076765866316No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGATATTTGTGGGAA17740.12053161644855698No Hit
ATCCTTGGGAGCAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGA17730.1204636730345499No Hit
GTGATGGTGAGGGCAGCTTTGCTCCCAAGGATGGAGCCAGAGAAGCGATC17650.11992012572249328No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17540.11917274816841542No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17520.11903686134040126No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17330.11774593647426677No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17310.1176100496462526No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17170.11665884185015352No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGCTCCCAAGGATGGAGCC17060.11591146429607566No Hit
CTCTCAGTGTCCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTAC16970.11529997357001195No Hit
CTAGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTCCCTCCAGGGGACACTGAGAGCGATGG16850.11448465260192701No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16810.1142128789458987No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16670.11326167114979961No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16590.11271812383774296No Hit
AACTAGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTC16540.11237840676770758No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGA16440.1116989726276368No Hit
CTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAA16380.11129131214359432No Hit
GGGCAGCTTTGCTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA16370.11122336872958725No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16070.10918506630937491No Hit
CTACTGGGGCTGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAATATCTATTATAGTGGCAGCAC16060.10911712289536782No Hit
GCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACACAGTAATAATCAGATTCATCATCTG16000.10870946241132536No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15990.10864151899731826No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15940.10830180192728288No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15890.1079620848572475No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA15540.10558406536699974No Hit
CCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGATA15400.10463285757090066No Hit
CCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGAGTGT15370.10442902732887942No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGACTGTGGTGACC15320.10408931025884403No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15240.1035457629467874No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15170.10307015904873786No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15150.10293427222072368No Hit
CAGTGTCCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGGC14950.10157540394058212No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14920.1013715736985609No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCA14850.10089596980051134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA100.008606377135.9001270
TACGGAA13950.062.83553370
GGTATCA9000.047.407161
GTGGTAT2400.044.088661
TGGTATC2600.040.6972272
CTACGGA14350.039.99981369
GTATCAA23400.039.0926781
CGCTAAT601.4141418E-534.24017732
TATCAAC27050.033.312952
TCTACGG15750.031.97669468
ATCAACG28550.031.562713
TCAACGC28900.031.1804664
GTGTCTA15700.030.94277465
GTCTACG15700.030.81584567
ATCGTTG450.004608344830.68670340
TGTCTAC15700.030.66890366
CAACGCA29500.030.6619875
TGGTCGA450.004797247730.4357132
GACGTGT15750.030.36267962
AACGCAG29900.030.2517976
Produced by FastQC (version 0.11.5)