FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471813
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACA63720.4329354340531032No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC59170.4020211806798826No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACACAGTAATAAT58470.3972651416993871No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA58090.3946832919671181No Hit
GATTATTACTGTGTGATATTTGTGGGAAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT56630.3847635535220847No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG53530.3637010951798904No Hit
GTCTATTACTGTGCGAGACGGTCTCCTGAACCAGGATATAATGGCTTTAAGGGCCGAGCCTTTGATATCTGGGGC50770.34494871291393675No Hit
GTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGG48280.32803080282617425No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG47040.31960581948929656No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46130.3134229688146524No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAG45710.3105693454263551No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35460.2409273460690998No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT35300.23984025144498655No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC35190.23909287389090866No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34770.23623925050261138No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33960.23073583396803804No Hit
GTAGATGAGCGTGCGTGGAGGCTGGCCTGGGGTCTGCTGGTACCAGCTGG33360.22665922912761335No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32280.21932134041484888No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31960.2171471511666224No Hit
CCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT31340.21293465949818352No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAGCTGCCCTCACCATCACGGGGGCCCAGG31240.21225522535811275No Hit
GTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCC30570.2077030166196385No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGACTGTGGTGACCCAGGAGCCAT29730.2019957698430439No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29040.19730767427655552No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGG28990.19696795720652013No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28950.1966961835504918No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28160.19132865384393263No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27830.18908652118169905No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGGCTGG27360.18589318072336636No Hit
CTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAACA27050.18378693488914694No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27020.18358310464712568No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGCTCTCAGTGTCCCCTGGAG26750.18174863246893458No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26430.17957444322070806No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC26350.17903089590865146No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTC25500.17325570571804977No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25230.17142123353985866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24910.16924704429163218No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24760.168227893081526No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23820.1618412121648606No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAGCAAAGCTGC22780.15477509710812448No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22660.15395977614003953No Hit
CCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTC22410.15226119078986258No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT22210.15090232250972102No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22030.1496793410575936No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21530.14628217035723967No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATT21510.1461462835292255No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21370.1451950757331264No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21170.14383620745298487No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGA20690.1405749235806451No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20650.1403031499246168No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTAGTAGAGACTGAG20620.14009931968259554No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20290.13785718702036195No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20090.1364983187402204No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG19750.13418824266397972No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19750.13418824266397972No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19520.13262554414181693No Hit
GTTTCTGGTGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGATTCG19430.13201405341575323No Hit
AGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTCCCTCCAGGGGACACTGAGAGCGATGGCTC19300.1311307890336612No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18850.12807333540334268No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18750.1273939012632719No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18560.1261029763971374No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18440.12528765542905246No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18390.12494793835901709No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18310.12440439104696044No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18260.12406467397692506No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18160.12338523983685427No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18110.12304552276681889No Hit
CAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCC17870.12141488083064901No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17680.12012395596451451No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGATATTTGTGGGAA17630.11978423889447913No Hit
ATCCTTGGGAGCAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGA17210.11693061550618183No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17160.11659089843614644No Hit
CACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTACCCC17130.1163870681941252No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17130.1163870681941252No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17090.1161152945380969No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16990.11543586039802611No Hit
GTGATGGTGAGGGCAGCTTTGCTCCCAAGGATGGAGCCAGAGAAGCGATC16560.11251429359572174No Hit
CTCTCAGTGTCCCCTGGAGGGACAGTCACACTTACCTGTGGCTTGACCTCTGGCTCAGTCTCTACTAGTTACTAC16360.11115542531558018No Hit
CTCTACTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGCCTCCACGCACGCTCATCTACAGCACAAA16250.11040804776150231No Hit
GGGCAGCTTTGCTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA16080.10925300972338198No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGCTCCCAAGGATGGAGCC16000.10870946241132536No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGA15940.10830180192728288No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT15930.1082338585132758No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGCGGATTCTCAGACTGTGGTGACC15900.10803002827125456No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15790.10728265071717671No Hit
AACTAGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTC15770.10714676388916256No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15670.10646732974909176No Hit
CTAGTAGAGACTGAGCCAGAGGTCAAGCCACAGGTAAGTGTGACTGTCCCTCCAGGGGACACTGAGAGCGATGG15410.10470080098490774No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15360.10436108391487234No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTAGACACGTCC15260.10368164977480156No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15220.10340987611877324No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15120.10273044197870246No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAAATGCCA15050.10225483808065292No Hit
CTACTGGGGCTGGATTCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGAATATCTATTATAGTGGCAGCAC15040.10218689466664584No Hit
GCTTGGTCCCTCCGCCGAACACCGAAATGCCACTTCCCACAAATATCACACAGTAATAATCAGATTCATCATCTG14800.10055625273047596No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC14750.10021653566044057No Hit
GTGTGATATTTGTGGGAAGTGGCATTTCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14730.1000806488324264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAA15650.058.7348970
GTGGTAT3350.051.8899231
TGGTATC3500.048.6822972
ATACCTT306.702538E-445.4429866
GGTATCA10700.043.6409721
GTATCAA27950.039.6332051
CTACGGA16300.037.5057169
TATCAAC31600.035.0481722
ATCAACG33450.033.003423
TCAACGC33650.032.8072664
CAACGCA34350.032.2400865
AACGCAG35100.031.6547286
TCTACGG17350.031.52919868
GTCTACG17300.030.16242667
ACGCAGA36900.030.1187867
CGCAGAG37000.030.042498
AGAGTAC37050.030.0111311
CAGAGTA37750.029.72063310
GCAGAGT37900.029.4210479
GTGTCTA17400.029.17811665
Produced by FastQC (version 0.11.5)