FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799554
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21050.2632717740140128No Hit
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20370.254767032620686No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20110.2515152197350023No Hit
GGCTAACACTTTCCCGTCCAGTTTCGGCGGAGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCAT19940.2493890343866706No Hit
GTAATACACACCCTTGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA18040.22562578637590458No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16190.20248788699700082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14990.1874795198323065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14650.1832271491356431No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGGCTGGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA12470.15596194878644845No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12320.15408590289086166No Hit
GATCTATGGAGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA12310.1539608331644892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12100.1513343689106677No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12080.1510842294579228No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12060.1508340900051779No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11790.14745720739312165No Hit
GGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGAGCATC11010.13770176873607037No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10960.1370764201042081No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.13682628065146318No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10930.13670121092509072No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10910.13645107147234584No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10460.13082293378558546No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10200.1275711208999017No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10070.12594521445705983No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACACTTTC10040.12557000527794246No Hit
GTGTATTACTGTGCGAGAGATAGGGTGGTGGTGACTTCCTACTACATGGA9960.12456944746696283No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9780.12231819239225869No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCC9550.11944158868569227No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9410.11769061251647793No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9400.11756554279010549No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9380.11731540333736058No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCCCTCCGCCGAAACTG9380.11731540333736058No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9140.11431372990442171No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9020.11281289318795229No Hit
CCCTTGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAAT8970.11218754455609004No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8850.11068670783962059No Hit
CAGTAATACACACCCTTGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATA8840.11056163811324812No Hit
GACATACATTATGCAAATTCTGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT8800.11006135920775832No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8590.10743489495393682No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8450.1056839187847225No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8320.1040580123418806No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT8310.10393294261550813No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC8240.10305745453090098No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8200.10255717562541117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)