FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799554
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22460.28090660543252866No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20430.2555174509789207No Hit
GTAATACACACCCTTGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA17750.22199876431110346No Hit
GGCTAACACTTTCCCGTCCAGTTTCGGCGGAGGGACCAAGGTGGAGATTAAACGAACTGTGGCTGCACCAT17360.2171210449825778No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15760.19710988876298538No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15380.19235723916083217No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15310.19148175107622498No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14720.1841026372202503No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12990.162465574557816No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11290.14120372107449902No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11280.14107865134812658No Hit
GATCTATGGAGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA11200.14007809353714695No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGGCTGGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAA10990.13745162928332544No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10680.13357446776577941No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10510.13144828241744771No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10430.1304477246064681No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10350.12944716679548848No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10250.12819646953176397No Hit
GGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGAGCATC9700.12131763458127906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9500.11881624005383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9350.11694019415824322No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9350.11694019415824322No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9310.1164399152527534No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCC9220.11531428771540132No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACACTTTC9020.11281289318795229No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACCTTGGTCCCTCCGCCGAAACTG8930.11168726565060019No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8850.11068670783962059No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8840.11056163811324812No Hit
CCCTTGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTGGAGATGGTGAAT8720.1090608013967787No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8540.10680954632207457No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8500.10630926741658474No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8470.10593405823746739No Hit
GTGTATTACTGTGCGAGAGATAGGGTGGTGGTGACTTCCTACTACATGGA8430.10543377933197758No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8150.10193182699354891No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8060.10080619945619683No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8050.10068112972982438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)