FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19830.28728846340512887No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAGTGTTACTGTCCCAGGCCTCACAGTAATAATCAG19460.28192806343236554No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA19020.2755535337350253No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16680.24165262579917043No Hit
CCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAAGATGAGGCTGATTATTACTGTGAGGC14930.21629938268474908No Hit
GATTATTACTGTGAGGCCTGGGACAGTAACACTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG14540.2106492313621066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14220.20601320976404097No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14150.20499908003946415No Hit
GTAATAATCAGCCTCATCTTCAAACTGGAGGTTGGAGATGGTGAGGTAGC14080.20398495031488725No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13630.19746554494260748No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13290.1925397719946628No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13190.19109101524526728No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12780.1851511125727457No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12600.1825433504238338No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12570.18210872339901513No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT12180.17645857207637267No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCG12100.17529956667685626No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC12050.17457518830215848No Hit
AGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCG11030.15979786945832436No Hit
GCCCCAGAGGTGGAGCCTGAGAAGCGATCAGGAACTCCGCTCCCCTTGTT10910.15805936135904974No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10210.14791806411328118No Hit
GTCCTTGATGAAGCTTGAGGGCAGTGGAACCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAC10180.14748343708846254No Hit
GTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG10100.14632443168894613No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9880.143137166840276No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9810.14212303711569915No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG9620.13937039929184772No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9380.1358933830932985No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9370.13574850741835892No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9340.13531388039354028No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9250.13400999931908433No Hit
GTCCCAGGCCTCACAGTAATAATCAGCCTCATCTTCAAACTGGAGGTTGG9240.13386512364414477No Hit
CTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACT9070.1314022371701724No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9070.1314022371701724No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACACCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGT8990.13024323177065603No Hit
GGGTCTCTCTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC8880.12864959934632098No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8710.1261867128723486No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT8330.12068143722464567No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACACCATCGCATGGCATC8330.12068143722464567No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8330.12068143722464567No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8180.11850830210055241No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8030.11633516697645914No Hit
GTATATTACTGTGCGAAGAACCTCCCCGACAATAGTGGGAGCTATTTGGG7990.11575566427670095No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7940.11503128590200319No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7830.11343765347766814No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT7770.11256839942803085No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7680.11126451835357488No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7440.10778750215502567No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC7390.10706312378032791No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7360.10662849675550926No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT7340.10633874540563015No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7300.10575924270587195No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7160.10373098325671824No Hit
GCACAGGGTCTCTCTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAG7050.10213735083238319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG951.2732926E-1163.61504770
CGTATGC800.060.49957741
TCTCGTA800.060.1804338
ACCGTAC800.059.5478327
TCGTATG850.056.86120240
TCCGAAC200.007261955651.9217942
CGTACTA1000.051.08173429
ATCTCGT900.049.6073237
GCCGTCT1050.046.45417846
CGTCAAC306.741433E-445.38622328
GACCGTA1050.045.35662526
GTACTAG1100.043.37669430
TACTAGA1050.042.2239731
GTGGTAT1800.041.5648541
TACACAT1500.040.809135
GGTATCA6100.040.6976281
ATGCCGT1200.040.50908744
TCTGGAG7700.039.2430570
GTATCAA16550.039.0419651
AATGGTA2251.8189894E-1235.81291270
Produced by FastQC (version 0.11.5)