FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18540.26859950133792687No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA18220.26396347973986123No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18040.26135571759094933No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCGAGTGTTACTGTCCCAGGCCTCACAGTAATAATCAG17850.2586030797670979No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16170.23426396637725336No Hit
CCTCTGGGGCTGACCGCTACCTCACCATCTCCAACCTCCAGTTTGAAGATGAGGCTGATTATTACTGTGAGGC14330.20760684218837605No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14190.20557858273922233No Hit
GATTATTACTGTGAGGCCTGGGACAGTAACACTCGGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG13960.20224644221561267No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13010.18848325309635536No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12910.18703449634695984No Hit
GTAATAATCAGCCTCATCTTCAAACTGGAGGTTGGAGATGGTGAGGTAGC12690.18384723149828974No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12470.1806599666496196No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12020.17414056127733984No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCCG11980.17356105857758167No Hit
GCTCTGGGGAGTCTGCACCATGGCCTGGACCCCACTCCTCCTCCTCTTCCCTCTCCTCCTCCACTGCACAGGGT11650.1687801613045765No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11580.1677660315799996No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11300.1637095126816922No Hit
AGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCG11110.16095687485784074No Hit
GTCCTTGATGAAGCTTGAGGGCAGTGGAACCTACAACAAGGGGAGCGGAGTTCCTGATCGCTTCTCAGGCTCCAC10610.1537130911108632No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC10600.15356821543592367No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10490.15197458301158862No Hit
GGGTCTCTCTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCAC10390.1505258262621931No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10290.1490770695127976No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10210.14791806411328118No Hit
GCCCCAGAGGTGGAGCCTGAGAAGCGATCAGGAACTCCGCTCCCCTTGTT9900.14342691819015513No Hit
GTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACTCTGAGCAGTGGG9860.14284741549039692No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9780.1416884100908805No Hit
CTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGTCAAGCTCACCTGCACT9740.1411089073911223No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9500.1376318911925731No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9330.13516900471860072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9160.13270611824462838No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9040.13096761014535377No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8560.12401357774825535No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAGCTACACCATCGCATGGCATCAGCAGCAGCCAGGGAAGGCCCCTCGGT8470.12270969667379937No Hit
GTCCCAGGCCTCACAGTAATAATCAGCCTCATCTTCAAACTGGAGGTTGG8460.12256482099885983No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8310.12039168587476656No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8290.12010193452488746No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT8230.11923268047525017No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7990.11575566427670095No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAGCTACACCATCGCATGGCATC7940.11503128590200319No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7910.11459665887718455No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT7900.11445178320224499No Hit
GTATATTACTGTGCGAAGAACCTCCCCGACAATAGTGGGAGCTATTTGGG7810.11314790212778904No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC7560.10952601025430028No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG7540.10923625890442118No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7390.10706312378032791No Hit
CCACTGCACAGGGTCTCTCTCCCAGCCTGTACTGACTCAATCATCCTCTGCCTCTGCTTCCCTGGGATCCTCGGT7200.10431048595647643No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7150.1035861075817787No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7120.10315148055696005No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6950.10068859408298768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGA200.00672372752.94270353
GGATGGG7300.043.08560670
GGTATCA6500.041.2528881
CGCGCTA504.272297E-640.76318715
TCTGGAG6350.039.84053870
GTATCAA16500.037.8507541
AATGGTA2251.8189894E-1236.46665670
TGGTATC2050.036.417912
AACGCTA400.002135888735.89830861
CTTAACG400.002209168935.65241258
GTGGTAT2100.035.5585481
ACGTTAA400.002433007134.95798548
TACGTTA400.002450873134.90588847
ATCAACG18750.032.939453
TCAACGC19100.032.513524
TATCAAC19100.032.335852
CAACGCA19450.031.9307565
TTAACGC450.00389982331.74902359
TGCTCGC652.5807625E-531.35402711
CGCTACT652.5846019E-531.34720817
Produced by FastQC (version 0.11.5)