FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112959
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45080.40504636738639965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38370.34475663523993244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30290.27215737506952187No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29810.2678445477326658No Hit
GGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC27630.24825712357777777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27450.24663981332645676No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26150.23495923928913823No Hit
GTAATAAACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA25670.23064641195228217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25360.22786104429722928No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24990.22453657322506937No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24930.22399746980796237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24850.22327866525181972No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24050.21609061969039292No Hit
CCTTAGTCCCTTGCCCGAACGTCCACCTACGGAAACTCTGTTGACAGTAG23350.20980107982414448No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGTATATTGCCACTTATTTAAATTGGTATCAGCAGAGA22420.20144497685898582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21960.19731185066116544No Hit
GACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATCCACTTTGCAA21780.19569454040984438No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21700.1949757358537017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20890.18769783972275708No Hit
AGTCAGTATATTGCCACTTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTG20120.1807793458698838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19790.17781427707579525No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19720.1771853230891704No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19610.17619696682447422No Hit
GTATATTGCCACTTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATC19220.17269279461327866No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCCCTTGCCCGAACGTC19180.17233339233520734No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19070.17134503607051113No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGTATATTGCCA18980.17053638094485063No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18790.16882922012401175No Hit
ACTTACTACTGTCAACAGAGTTTCCGTAGGTGGACGTTCGGGCAAGGGAC18700.16802056499835125No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18660.1676611627202799No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18650.16757131215076207No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18420.16550474905185186No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17580.15795730121235374No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17480.1570587955171754No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17440.15669939323910406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17340.15580088754392568No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17250.15499223241826518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCC17140.154003876153569No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16440.14771433628732056No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16410.14744478457876706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16070.14438986521516067No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15640.14052629072589376No Hit
GTTTATTACTGTGCGAGAGAGGTGGGGGCATATAGTGGCTACGAGTGCGA15540.1396277850307154No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG15450.1388191299050549No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15300.13747137136228738No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15230.13684241737566255No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15040.13513525655482367No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14660.13172093491314593No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14420.1295645212447179No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC14280.12830661327146822No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA14250.12803706156291472No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14170.12731825700677205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14150.12713855586773637No Hit
CTATATGCCCCCACCTCTCTCGCACAGTAATAAACGGCCGTGTCCTCGAC14150.12713855586773637No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14150.12713855586773637No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14110.12677915358966502No Hit
GTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGTCGAGGACACGGCCGTTTATTA13960.1254313950468975No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13940.12525169390786184No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13780.12381408479557648No Hit
GTATTGGATGACCTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGT13680.12291557910039813No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13640.12255617682232678No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13440.12075916543197009No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13210.11869260233305988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13210.11869260233305988No Hit
ATACTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT13120.11788394720739936No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13120.11788394720739936No Hit
GCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGTT13030.11707529208173886No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGGTATTGGATGACCTGGGTCCGTCAGGCTCCAGGGAAGGGG12970.11653618866463186No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12900.11590723467800701No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12750.11455947613523948No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG12490.11222336132777579No Hit
TCCCTGGTCTCTGCTGATACCAATTTAAATAAGTGGCAATATACTGACTTGCCCGGCAAGTGATGGTGACT12460.11195380961922229No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12140.10907859139465156No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12010.10791053399091971No Hit
CTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACTCTCA11800.10602367203104517No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11760.10566426975297384No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11690.10503531576634899No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11580.10404695950165281No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11520.1035078560845458No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGT11500.10332815494551012No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11500.10332815494551012No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11480.10314845380647444No Hit
GTTCCTGATCTCTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGT11390.10233979868081394No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11380.10224994811129609No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11320.10171084469418909No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCCGT11230.10090218956852859No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11140.10009353444286806No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCAGTGAACCTTGACGGGACCCCG11130.10000368387335024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGT150.002244578269.8444746
GTGGTAT1700.051.6176451
TCGTATG551.4695615E-743.90952740
GTACGAC350.001501876338.5696953
CGTACTA558.410965E-636.93602429
ATCTCGT655.7372927E-736.91175537
GTATCAA18000.035.6251
CGTATGC601.322666E-534.5749941
CTAGATC601.465169E-534.06224433
ACCGTAC601.539522E-533.8168627
GACCGTA601.5466543E-533.79403726
GTCGGGT400.002889143533.7560720
ACGAGAC701.1544926E-633.75606522
TATGTAG400.002894876533.7424167
TATCAAC20200.032.244842
GATCTCG751.7753955E-631.94645136
AAGGCGT450.00381531431.89318557
GGTACGT500.00405310131.50141368
CCTACCG450.00411041331.411551
TGTGCTA652.6985155E-531.15244719
Produced by FastQC (version 0.11.5)