FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112959
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44130.39651056328220535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31800.285724811066715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31790.2856349604971971No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28860.2593087436284715No Hit
GGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC27290.2452022042141714No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26770.24052997459924397No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26740.24026042289069047No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25970.23334192903781723No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24210.21752822880267828No Hit
GTAATAAACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA24080.21636017139894642No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23010.2067461604605381No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGTATATTGCCACTTATTTAAATTGGTATCAGCAGAGA22530.20243333312368203No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21850.19632349439646923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21320.19156141421202397No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21040.1890455982655246No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20580.1849124720677042No Hit
CCTTAGTCCCTTGCCCGAACGTCCACCTACGGAAACTCTGTTGACAGTAG20460.18383426523349017No Hit
AGTCAGTATATTGCCACTTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTG20250.18194740327361564No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19710.17709547251965255No Hit
GACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATCCACTTTGCAA19680.17682592081109905No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19510.17529846112929587No Hit
GTATATTGCCACTTATTTAAATTGGTATCAGCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATC19280.17323189803038566No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18900.16981757638870792No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18870.16954802468015442No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGTATATTGCCA18730.16829011670690475No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18160.16316863424438816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18160.16316863424438816No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18130.16289908253583466No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCCCTTGCCCGAACGTC18120.1628092319663168No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17750.15948476089415692No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17470.15696894494765756No Hit
ACTTACTACTGTCAACAGAGTTTCCGTAGGTGGACGTTCGGGCAAGGGAC17020.15292566931935497No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17010.15283581874983715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16600.1491519453996059No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16500.14825343970442756No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16300.14645642831407085No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16200.14555792261889253No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCC16060.14430001464564285No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15840.14232330211625047No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15700.14106539414300076No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15570.1398973367392689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15550.13971763560023326No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15000.13477585427675234No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA14940.13423675085964532No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14870.13360779687302046No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14730.13234988889977078No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14600.13118183149603893No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14530.1305528775094141No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14160.1272284064372542No Hit
GTTTATTACTGTGCGAGAGAGGTGGGGGCATATAGTGGCTACGAGTGCGA14120.12686900415918287No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14070.12641975131159366No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGGTATTGGATGACCTGGGTCCGTCAGGCTCCAGGGAAGGGG13970.12552124561641534No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13950.12534154447737966No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC13780.12381408479557648No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13750.12354453308702298No Hit
GTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGAGAGTCGAGGACACGGCCGTTTATTA13740.12345468251750513No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13670.12282572853088031No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13490.12120841827955926No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG13470.12102871714052359No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13470.12102871714052359No Hit
GTATTGGATGACCTGGGTCCGTCAGGCTCCAGGGAAGGGGCTGGAGTGGT13460.12093886657100576No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13140.11806364834643504No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12940.11626663695607833No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12740.11446962556572166No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12710.11420007385716814No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12650.11366097044006114No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12580.11303201645343629No Hit
CTATATGCCCCCACCTCTCTCGCACAGTAATAAACGGCCGTGTCCTCGAC12500.11231321189729361No Hit
ATACTGACTTGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCAT12370.11114515449356176No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12290.11042634993741907No Hit
TCCCTGGTCTCTGCTGATACCAATTTAAATAAGTGGCAATATACTGACTTGCCCGGCAAGTGATGGTGACT12260.11015679822886557No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12120.10889889025561589No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGT12010.10791053399091971No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11970.10755113171284836No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11690.10503531576634899No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11660.10476576405779549No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCAGTGAACCTTGACGGGACCCCG11600.10422666064068847No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11580.10404695950165281No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11560.10386725836261713No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTTCCGT11430.10269920095888527No Hit
GTTCCTGATCTCTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGT11400.10242964925033177No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11350.10198039640274259No Hit
GCAGAGACCAGGGAAAGCCCCTAAGTTCCTGATCTCTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGTT11330.10180069526370694No Hit
CTGCTGCATCCACTTTGCAAAGCGGGGTCCCGTCAAGGTTCACTGGCAGTGGATCTGGGACAGATTTCACTCTCA11310.10162099412467127No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11280.10135144241611777No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11280.10135144241611777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)