FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124122
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTGCAAAGGAGAGTGATGATAGTACTGGTTCTTACGGGTTAATTGACTACTGGGGCCAGGGAACC35680.31740327117519274No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35340.31437868843417355No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32870.29240598440382803No Hit
GTAATACAGGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA32570.2897372349264582No Hit
GTATTACTGTCATCAGAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGA29200.259758282464003No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT28250.2513072424523317No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25330.22533141420593136No Hit
CAGTAATACAGGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA24870.22123933167396423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23660.2104753754485723No Hit
CAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA23420.20834037586667642No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23320.20745079270755312No Hit
GAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT22100.19659787816624885No Hit
CTGTATTACTGTGCAAAGGAGAGTGATGATAGTACTGGTTCTTACGGGTTAATTGACTACTGGGGCCAGGGAACC19780.17595954887458834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19720.17542579897911437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19460.1731128827653938No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGACAGGTGATGGTGACTT19220.17097788318349788No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19190.1707110082357609No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGCAGTTTA19090.16982142507663758No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18920.168309133706128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18650.16590725917649507No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17940.15959121874671967No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17920.159413302114895No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17750.1579010107443854No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17730.15772309411256075No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17490.15558809453066483No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16800.14944997073271407No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15870.1411768473528674No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15800.1405541391414811No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15710.1397535142982701No Hit
GTCTCCAGACTCTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT15650.13921976440279613No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG15400.1369958065049879No Hit
CTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTCTCTTATTAATTGGGATGGTGGTAG15300.13610622334586459No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14950.13299268228893307No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAGAGGTAAA14590.12979018291608918No Hit
GTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGTCGGGCCAGTCAGAGCATTGGTAGTAGCTTACACTGGTA14550.12943434965243986No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14480.12881164144105356No Hit
GCGTATTACTGTCATCAGAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14260.1268545584909823No Hit
CTCTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGTCGGGCCAGTCAGAGCATTGGTAGTAGCTTACA14220.126498725227333No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT13900.12365205911813842No Hit
CTGCTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC13900.12365205911813842No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13700.12187289279989183No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13700.12187289279989183No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13680.12169497616806715No Hit
CTCCAGACCCTTCCCCGGAGTTTGACGGACCCAGTGCATGGAGTCTTCAT13650.12142810122033017No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG13650.12142810122033017No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13550.12053851806120687No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGATGAAGACTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGT13520.12027164311346988No Hit
GTCTGAGAACTGAGGACACCGCCCTGTATTACTGTGCAAAGGAGAGTGAT13320.1184924767952233No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13270.11804768521566165No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG13250.11786976858383699No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13170.11715810205653834No Hit
GGTCTGGAGTGGGTCTCTCTTATTAATTGGGATGGTGGTAGCACATTCTA13010.11573476900194107No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12880.11457831089508079No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12810.11395560268369447No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12650.1125322696290972No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12620.11226539468136021No Hit
GTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA12370.11004143678355197No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC12320.10959664520399032No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12240.10888497867669168No Hit
GGACTGGGAAGCAAACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT12190.10844018709713003No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12170.10826227046530537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11860.10550456267202314No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11860.10550456267202314No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11740.1044370628810752No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGCCTGGAAG11630.10345852140603957No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11560.10283581319465325No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11550.10274685487874091No Hit
GTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACCCTCACCATCAATAGC11320.10070081361275734No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11310.100611855296845No Hit
GGCCAAAAGTGAGAGGTAAACTGCTACTCTGATGACAGTAATACGCTGCA11300.10052289698093267No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA11280.100344980349108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2050.051.621451
TCTTATA1050.045.515692
GGTATCA8350.039.655881
GTATCAA21550.039.4434131
TGGTATC2700.039.1940652
CTATACC451.0659537E-437.9297374
ACCGTAC1450.035.3642427
TATCAAC24350.034.767632
CGTATGC1600.034.52222441
GACCGTA1500.034.17325226
ATAGTCT400.002733415534.1398056
TATAGTC400.00273460834.136775
ATCAACG25000.033.863673
TCGTATG1650.033.4324840
CGTACTA1550.033.09741629
TCAACGC25850.032.7501684
CAACGCA25950.032.6239625
ATGCCGT1700.032.59414344
ACGAGAC1700.032.137322
TACTAGA1600.032.0917331
Produced by FastQC (version 0.11.5)