FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124122
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTGCAAAGGAGAGTGATGATAGTACTGGTTCTTACGGGTTAATTGACTACTGGGGCCAGGGAACC33420.2972986917790062No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33360.2967649418835322No Hit
GTAATACAGGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA28040.24943911781817274No Hit
GTATTACTGTCATCAGAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGA27490.2445464104429946No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25540.2271995388400903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25170.2239080811513341No Hit
AGCTTACACTGGTACCAGCAGAAACCAGATCAGTCTCCAAAGCTCCTCAT24810.22070558177849023No Hit
CAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA23230.2066501678643421No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22330.19864391943223242No Hit
CAGTAATACAGGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA20920.18610079688859393No Hit
GAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20810.1851222554135583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20730.18441058888625966No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19880.17684913203371164No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18620.1656403842287581No Hit
CTGTATTACTGTGCAAAGGAGAGTGATGATAGTACTGGTTCTTACGGGTTAATTGACTACTGGGGCCAGGGAACC18400.16368330127868685No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18400.16368330127868685No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17530.15594392779431412No Hit
GTGTAAGCTACTACCAATGCTCTGACTGGCCCGACAGGTGATGGTGACTT17290.1538089282124182No Hit
GATTTCACCCTCACCATCAATAGCCTGGAAGCTGAAGATGCTGCAGCGTATTACTGTCATCAGAGTAGCAGTTTA17280.15371996989650588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16830.14971684568045104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16210.14420143009388658No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15780.14037622250965642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15720.13984247261418245No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15300.13610622334586459No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15090.13423809871170567No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14990.13334851555258237No Hit
CTGCTACTCTGATGACAGTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC14830.1319251824979851No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14650.13032393281156315No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14500.1289895580728782No Hit
CTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTCTCTTATTAATTGGGATGGTGGTAG14470.1287226831251412No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14290.12712143343871926No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14270.12694351680689464No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13850.12320726753857678No Hit
GGGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTCGAGGGGACCCCT13840.12311830922266444No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGAGAGGTAAA13760.1224066426953658No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13650.12142810122033017No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13610.12107226795668087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13570.12071643469303153No Hit
GTCTCCAGACTCTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCT13290.11822560184748632No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTTGATGAAGACTCCATGCACTGGGTCCGTCAAACTCCGGGGAAGGGT13200.11742497700427533No Hit
GTCTGAGAACTGAGGACACCGCCCTGTATTACTGTGCAAAGGAGAGTGAT13200.11742497700427533No Hit
CTCTCAGTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGTCGGGCCAGTCAGAGCATTGGTAGTAGCTTACA13160.11706914374062602No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13130.11680226879288902No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12970.11537893573829175No Hit
GAGTTTGGACTGAGCTGGGTTTTCCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAAT12880.11457831089508079No Hit
GTCTGTGACTCCAAAGGAGAAAGTCACCATCACCTGTCGGGCCAGTCAGAGCATTGGTAGTAGCTTACACTGGTA12730.11324393615639583No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12500.11119789489041226No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTCGCCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG12270.10915185362442867No Hit
GCGTATTACTGTCATCAGAGTAGCAGTTTACCTCTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12160.10817331214939305No Hit
GTAATACGCTGCAGCATCTTCAGCTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA12090.10755060393800672No Hit
CTCCAGACCCTTCCCCGGAGTTTGACGGACCCAGTGCATGGAGTCTTCAT12040.10710581235844507No Hit
GGCCAAAAGTGAGAGGTAAACTGCTACTCTGATGACAGTAATACGCTGCA12000.10674997909479578No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11950.10630518751523411No Hit
GGTCTGGAGTGGGTCTCTCTTATTAATTGGGATGGTGGTAGCACATTCTA11940.10621622919932178No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA11670.10381435466968887No Hit
TCCCAGTCCTTCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGG11540.10265789656282859No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11520.10247997993100393No Hit
GGACTGGGAAGCAAACTTGATGAGGAGCTTTGGAGACTGATCTGGTTTCT11470.10203518835144228No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11340.10087873024458198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)