FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613845
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29520.48090315959240526No Hit
GTATTAATACTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTCGACTCCTGATCTATCAGGCGTCTA28950.471617427852308No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24740.40303333903509847No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21290.34683022587135187No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18940.30854694589024917No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.29111583543076835No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17450.28427371730648615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17340.2824817339882218No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16240.26456190080557795No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14660.2388225040523259No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14360.2339352768206958No Hit
CACCATCACCAGCCTGCAGCCTGATGACTTTGCAACTTACTACTGCCAACAATATGATGCTTATTGGACATTCGG13960.22741897384518894No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13760.2241608223574355No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACTTTGCAACTTA13510.22008813299774374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12980.21145403155519715No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12780.20819588006744374No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12750.20770715734428075No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12440.20265702253826293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12020.19581490441398075No Hit
GTATTAATACTTTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTATAGATGCAGTCAGGGTGGAAGGAGA11990.19532618169081772No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11820.19255675292622731No Hit
CCTTCCACCCTGACTGCATCTATAGGAGACACAGTCACCATCACTTGCCG11780.19190512262867662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11720.1909276771823506No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11420.18604044995072047No Hit
ACTTACTACTGCCAACAATATGATGCTTATTGGACATTCGGCCGAGGGAC11080.18050159242153963No Hit
CTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACT10850.1767547182106232No Hit
ATATGATGCTTATTGGACATTCGGCCGAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT10790.1757772727642972No Hit
GACCCAGGCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGG10660.17365947429725745No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC10660.17365947429725745No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTCGGCCGAATGTC10160.1655140955778739No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9860.16062686834624376No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9800.1596494228999177No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9690.15785743958165335No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9570.1559025486890013No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9360.1524814896268602No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9340.15215567447808487No Hit
AGGCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC9330.15199276690369717No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8950.1458022790769657No Hit
GGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8610.14026342154778487No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8260.1345616564442164No Hit
GTCTCCTTCCACCCTGACTGCATCTATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAATA8110.1321180428284013No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7920.12902279891503554No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7850.12788244589432185No Hit
ATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAATACTTGGTTGGCCTGGTATCAGCAAAAA7820.12739372317115885No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7810.12723081559677119No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7790.12690500044799582No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7720.12576464742728213No Hit
GTAGTAAGTTGCAAAGTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCG7710.12560173985289447No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGGTGACCCAGTCTCCTTCCACC7680.12511301712973144No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7670.12495010955534379No Hit
GCATCATATTGTTGGCAGTAGTAAGTTGCAAAGTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC7640.12446138683218076No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7590.1236468489602424No Hit
CTCCTGATCTATCAGGCGTCTACTTTAGAAACCGGGGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGA7510.12234358836514105No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7490.1220177732163657No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7380.12022578989810132No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7320.11924834445177529No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCGCTGAACCTTGATGGGACCCCG7310.11908543687738761No Hit
GTCCAATAAGCATCATATTGTTGGCAGTAGTAAGTTGCAAAGTCATCAGG7300.11892252930299994No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7290.11875962172861226No Hit
TCACCAGCCTGCAGCCTGATGACTTTGCAACTTACTACTGCCAACAATAT7210.1174563611335109No Hit
GTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCGCTGAACCTTGATGGGAC7060.11501274751769583No Hit
GTCCCAGAGCCACTGCCGCTGAACCTTGATGGGACCCCGGTTTCTAAAGT6880.11208041117871775No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6780.11045133543484105No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6740.10979970513729036No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6720.10947388998851501No Hit
CATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACTTTG6710.10931098241412736No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6670.10865935211657667No Hit
ACCTTGATGGGACCCCGGTTTCTAAAGTAGACGCCTGATAGATCAGGAGTCGAGGGGCTTTCCCTGGTTTTTG6660.10849644454218899No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6660.10849644454218899No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6640.10817062939341365No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6600.10751899909586296No Hit
CTATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAAT6560.10686736879831227No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6430.10474957033127257No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6390.10409794003372187No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6310.10279467943862049No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6290.10246886428984517No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6230.10149141884351913No Hit
CACCATCACTTGCCGGGCCAGTCAAAGTATTAATACTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCC6180.10067688097158077No Hit
ACTTTAGAAACCGGGGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGACTTCACTCTCACCATCACCAGC6160.10035106582280542No Hit
GCCTGATGACTTTGCAACTTACTACTGCCAACAATATGATGCTTATTGGA6150.10018815824841777No Hit
CAATATGATGCTTATTGGACATTCGGCCGAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT6140.10002525067403009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)