FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613845
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29990.48855981558862577No Hit
GTATTAATACTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTCGACTCCTGATCTATCAGGCGTCTA26730.43545194633824497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21660.3528578061236957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20000.3258151487753423No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19080.31082765193167655No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16500.2687974977396574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16360.26651679169823No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.2653764386775163No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14300.23295783137436973No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13860.22578989810131223No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13790.22464954508059853No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13480.21959941027458071No Hit
CACCATCACCAGCCTGCAGCCTGATGACTTTGCAACTTACTACTGCCAACAATATGATGCTTATTGGACATTCGG13250.21585253606366425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13010.21194275427836015No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACTTTGCAACTTA13000.2117798467039725No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12850.20933623308815744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12150.19793270288102047No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12000.19548908926520536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11900.19386001352132867No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11060.1801757772727643No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10880.17724344093378622No Hit
GTATTAATACTTTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTATAGATGCAGTCAGGGTGGAAGGAGA10830.17642890306184786No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10700.17431110459480814No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10630.17317075157409445No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10590.17251912127654376No Hit
GTCCCAGAGCCACTGCCGCTGAACCTTGATGGGACCCCGGTTTCTAAAGT10490.17089004553266704No Hit
CTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACT10400.169423877363178No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC10370.168935154640015No Hit
GACCCAGGCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGG10060.1638850198339972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9920.1616043137925698No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCGCTGAACCTTGATGGGACCCCG9880.1609526834950191No Hit
CCTTCCACCCTGACTGCATCTATAGGAGACACAGTCACCATCACTTGCCG9820.15997523804869307No Hit
ACTTACTACTGCCAACAATATGATGCTTATTGGACATTCGGCCGAGGGAC9810.1598123304743054No Hit
ATATGATGCTTATTGGACATTCGGCCGAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT9670.15753162443287802No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8960.14596518665135336No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8700.14172958971727392No Hit
GGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8620.14042632912217254No Hit
AGGCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC8620.14042632912217254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8580.13977469882462185No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8410.13700527006003144No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTCGGCCGAATGTC8400.13684236248564377No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8280.13488747159299172No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8100.13195513525401364No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8030.13081478223329995No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7980.1300002443613616No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7760.1264162777248328No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7750.12625337015044513No Hit
GTAGTAAGTTGCAAAGTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCG7670.12495010955534379No Hit
GTCTCCTTCCACCCTGACTGCATCTATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAATA7650.12462429440656844No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7580.12348394138585474No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7560.12315812623707939No Hit
CTCCTGATCTATCAGGCGTCTACTTTAGAAACCGGGGTCCCATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGA7370.12006288232371365No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGGTGACCCAGTCTCCTTCCACC7270.11843380657983692No Hit
GTCCAATAAGCATCATATTGTTGGCAGTAGTAAGTTGCAAAGTCATCAGG7010.11419820964575747No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7010.11419820964575747No Hit
GTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGAGCCACTGCCGCTGAACCTTGATGGGAC6920.11273204147626845No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6900.1124062263274931No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6890.11224331875310542No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6790.11061424300922872No Hit
TCACCAGCCTGCAGCCTGATGACTTTGCAACTTACTACTGCCAACAATAT6770.11028842786045337No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6750.10996261271167802No Hit
GCATCATATTGTTGGCAGTAGTAAGTTGCAAAGTCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC6730.10963679756290268No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6640.10817062939341365No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6630.10800772181902597No Hit
CATCAAGGTTCAGCGGCAGTGGCTCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGACTTTG6560.10686736879831227No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6550.10670446122392462No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6540.10654155364953694No Hit
ATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAAAGTATTAATACTTGGTTGGCCTGGTATCAGCAAAAA6530.10637864607514927No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6490.10572701577759858No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT6480.10556410820321091No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6360.10360921731055886No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6340.10328340216178351No Hit
GTATCAACGCAGAGTACGGGGTCAGACCCAGGCAGGACACAGCATGGACA6230.10149141884351913No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6210.10116560369474378No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6180.10067688097158077No Hit
GCCTGATGACTTTGCAACTTACTACTGCCAACAATATGATGCTTATTGGA6170.10051397339719309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA150.00255484267.597187
AGTACGA200.00793870550.76407619
GCTACAA3450.041.1159570
GTATCAA15300.037.3361241
GCACGGT400.00255795734.60224542
CTATTAT400.002869224633.8013461
AGTCGTG400.002870371333.798597
CCTTATC400.002873813333.790332
TATCAAC17800.031.3224962
ATCAACG17700.031.3060043
ATCTCAC450.00419451131.27948850
TCAACGC18000.030.5965274
CAACGCA18150.030.5273325
GGCTACA3050.030.19566769
AACGCAG18950.029.0602996
GTGGTAT1750.028.972581
ACGGGAC1302.4920155E-1028.61513316
CGCAGAG19400.028.404738
CGAACGT500.006722769228.40215754
TCGAACG500.006790491328.34392553
Produced by FastQC (version 0.11.5)