FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032310
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35100.3400141430384284No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31990.3098875337834565No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCCACATTTTCTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAG30630.2967131966172952No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC28600.27704856099427494No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG28530.2763704701107226No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAGCAGTAATAATCAG26650.25815888638102896No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAAT26340.25515591246815394No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT23320.22590113434917805No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23210.22483556296073853No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22800.22086388778564578No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATAT22730.22018579690209336No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG22470.21766717362032723No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22230.21534229059100465No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG22050.21359862831901272No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21990.21301740756168203No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC21830.21146748554213365No Hit
GGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC21370.20701145973593205No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20870.2021679534248433No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20860.20207108329862153No Hit
GCCCTCAGGGGTCCCTGATCGATTCTCTGGCTCCAAGTCTGGCAACACGG20800.20148986254129087No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19080.18482820083114568No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18720.1813408762871618No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18630.18046904515116585No Hit
CCCCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA18530.1795003438889481No Hit
GTCTTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA18480.1790159932578392No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18360.1778535517431779No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAACA18180.17610988947118597No Hit
GTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGGGCTGGATCCG18130.17562553884007712No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGC17900.1733975259369763No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17790.17233195454853675No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA17580.1702976818978795No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17010.16477608470323837No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16680.16157937053791982No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16590.16070753940192384No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15810.15315166955662543No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15750.1525704487992948No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15690.15198922804196413No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15540.1505361761486375No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC15480.14995495539130688No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15380.1489862541290891No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15290.14811442299309316No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATGAG14850.1438521374393351No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14250.13803992986602862No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14010.13571504683670604No Hit
CCTGAGGGCCGCTCATTGACATCATAAATCATGAGTTTGGGGGCTTTGCC13980.1354244364580407No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13750.13319642355493988No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA13740.1330995534287181No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGA13650.13222772229272214No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13570.13145276128294794No Hit
ATCCTGGGCTCAATCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG13400.1298059691371778No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13230.1281591769914076No Hit
GCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAGCAGTAATAATCAGCCTCATCTTCAGC13200.1278685666127423No Hit
GACCATCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCTGCTCATATGCAGGCAGCCACATTTTCTT13180.12767482636029873No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGC12760.12360628105898422No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCT12600.12205635903943582No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12580.12186261878699227No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12470.12079704739855275No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12460.12070017727233098No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAAAATGTGGCTGCCTGCATATGAGCAGCAGTAATAATCAGC12460.12070017727233098No Hit
CCTCAGGGGTCCCTGATCGATTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG12400.12011895651500035No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12140.11760033323323421No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT11980.11605041121368581No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11940.11566293070879871No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11890.11517858007768983No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11800.11430674894169386No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11800.11430674894169386No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11760.11391926843680676No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAATCGATCAGGGACCCCTGAGGGCCGCT11720.11353178793191968No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAACAGGAGTGGTTACTCCTGGG11690.11324117755325436No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11690.11324117755325436No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11600.11236934641725839No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11530.11169125553370596No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11510.11149751528126241No Hit
CTATAAGACATACTCCCTATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCCCCAGGAGTAACCACTC11480.11120690490259708No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACT11340.10985072313549225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11040.10694461934883902No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10920.10578217783417772No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10770.1043291259408511No Hit
GTATTACTGTGGGAGACACATTTATGGTTCATACTACTACTACTACTACA10700.10365103505729867No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGG10610.10277920392130271No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAATCGATC10590.10258546366885916No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10580.10248859354263738No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10420.10093867152308898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACCG100.0075656627141.8831570
GGTCGTA306.087765E-446.3375547
ATCGGGA306.5563706E-445.6455121
GGTATCA8250.045.241521
CCATCGG405.238264E-542.78851319
CCTATTG350.001231477440.1587357
GTGGTAT2050.040.0887031
GGTGTAT350.001398322539.134211
TCGGGAT350.001400323839.12282622
TGGTATC2200.038.887342
CGTAAAC459.427113E-538.7290150
TACCGAT7400.038.34679470
GTATCAA21350.038.332281
CTATTGT400.002358295535.18266358
TCGTAAA400.002479459434.8251649
GTCGTAA400.002496905234.77541748
TATCAAC23350.034.5872462
CATCGGG400.002697226134.2324720
TAAACCG400.002707501934.2059365
ATCAACG25450.031.589643
Produced by FastQC (version 0.11.5)