FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002356
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG36850.36763385463847176No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23120.23065657311374402No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19400.19354401031170562No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18470.184265869611196No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18000.17957691678405677No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16720.16680700270163495No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15590.15553356292574694No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15460.15423661852675097No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15300.15264037926644825No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGATGATCACTACTACTATCCCACAC14910.14874954606946034No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14760.14725307176292654No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14550.14515800773377924No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14520.14485871287247246No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGATGATCACTA14250.1421650591207116No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13740.13707704647849667No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13630.13597963198703852No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13560.1352812773106561No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13310.13278715346643308No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12960.12929538008452085No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA12880.12849726045436952No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG12800.12769914082421815No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12770.12739984596291137No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12670.12640219642522219No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12090.12061582910662479No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12070.12041629919908696No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11700.11672499590963689No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA11660.11632593609456122No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG11500.11472969683425849No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11490.11462993188048956No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT11210.11183651317495978No Hit
GTAATACAAGGCCGTGTCCTCAACTCTCAGACTGTTCATTTGCAGATACA11160.11133768840611519No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC11030.11004074400711922No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA10850.10824497483927867No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10810.10784591502420297No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG10560.10535179117997996No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10390.10365578696590833No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA10120.10096213321414747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT251.6212506E-460.5779667
TACGCTA251.7244315E-459.8268666
ACGACTT200.007530644551.4485938
TTCGCAT400.00937696448.66858770
GGTATCA11400.041.0926131
GTGGTAT3750.037.7460751
GGATGGG6800.037.2171570
ATACGCT400.001802299537.1634565
TATACGC400.001843572736.99249664
CGTATGC852.93403E-936.57291441
GTATCAA28500.036.06691
TCGTATG905.2241376E-934.4758640
TACGTAT951.9737126E-434.15339370
TGGTATC4050.034.1179282
TATCAAC32650.031.1729262
ATCAACG33700.030.101663
TCAACGC34050.029.891224
CAACGCA35200.029.0104055
ACCGTAC1052.8598151E-828.955227
CGGACCG704.517074E-528.8873025
Produced by FastQC (version 0.11.5)