FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834045
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34820.4174834691173738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31020.37192237828894126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25430.30489961572816815No Hit
GTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCC23790.2852364081074762No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22990.27564459951201675No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22590.270848695214287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22110.2650936100570113No Hit
GGCACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT21330.2557415966764383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20390.24447122157677342No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAATGCAGTGCCTGACCCACTGCCG20230.24255285985768155No Hit
GCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTAGTCAAAGTCCCGTA20010.2399151124939302No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19970.23943552206415722No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19650.23559879862597344No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18640.22348914027420583No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC18590.22288965223698962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17900.21461671732340581No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17840.21389733167874636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17840.21389733167874636No Hit
GGCCAAAAGTGTACGGCCAGTGTGTGCCTTGCATGCAGTAATAAACCCCA17650.21161927713732473No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17240.20670347523215174No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17150.20562439676516256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16050.19243565994640577No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGCATTCACACTGAA15560.18656067718168684No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC15530.1862009843593571No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15180.1820045680988436No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15130.18140508006162737No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.1751704044745787No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGA14510.1739714284001463No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA14370.17229286189594087No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14130.16941531931730303No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG13610.16318064373025434No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGT13390.160542896366503No Hit
GTCCAAGGCTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC13360.16018320354417329No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12800.15346893752735163No Hit
GGGTCAGGCACTGCATTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGA12760.15298934709757867No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12590.15095108777104352No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12500.14987200930405434No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12490.1497521116966111No Hit
GTTTATTACTGCATGCAAGGCACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12450.14927252126683813No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12370.1483133404072922No Hit
GTCTCCACTCTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTAGTCAAAGTCCCGTATC12110.14519600261376783No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC11990.14375723132444893No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11920.14291794807234623No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11100.13308634426200025No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11080.13284654904711377No Hit
GTATTACTGTGGAAAAGATGTGTTGCCTGGAGGTCTGGACGTCTGGGGCC11030.13224706100989755No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT10840.12996900646847592No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10620.12733125910472456No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10500.12589248781540566No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10470.1255327949930759No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10430.12505320456330293No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGATTCCATCATAGGAT10390.12457361413352996No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10390.12457361413352996No Hit
GCATTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGCACACACTGG10380.12445371652608672No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10120.12133637873256238No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10100.1210965835176759No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9760.11702006486460563No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9700.11630067921994616No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9540.11438231750085429No Hit
GTGTACGGCCAGTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCTTC9490.11378282946363806No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9390.11258385338920561No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC9380.11246395578176237No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9360.11222416056687588No Hit
CTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTA9340.1119843653519894No Hit
CCACTCTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAG9270.11114508209988669No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9220.11054559406267048No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9180.11006600363289751No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8990.10778794909147588No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8940.10718846105425966No Hit
CTGCATTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGCACACA8910.10682876823192994No Hit
CAGTAATACAGGGCCGAATCCTCAGGTCTCAGACTGTTCATTTCCAGATA8890.10658897301704345No Hit
GTCTGAGACCTGAGGATTCGGCCCTGTATTACTGTGGAAAAGATGTGTTG8880.10646907540960021No Hit
GTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT8830.105869587372384No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8720.10455071369050829No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8710.10443081608306505No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8670.10395122565329208No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8650.1037114304384056No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8560.1026323519714164No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8450.10131347828954074No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8410.10083388785976775No Hit
GTAATACAGGGCCGAATCCTCAGGTCTCAGACTGTTCATTTCCAGATAGA8400.10071399025232451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCCG200.006661976753.06736855
ACTCGGT200.00781327550.97063412
GTGGTAT2200.044.7896921
TGGTATC2150.044.2509232
GGTATCA7500.042.5862461
GTATCAA19350.038.280561
AATGTCG400.00227443735.44240656
GACAGGT2250.034.7334631
TATCAAC21600.034.2930032
AATTTCG400.002765335634.05801828
ATTCACT2350.033.2554442
CGTATGC1154.7293724E-1133.02173241
ATCTCGT1056.8030204E-1032.681737
ATCAACG23150.031.9969273
TCAACGC23150.031.9969274
TCACTTT2550.031.9796584
GTTGGCG450.00412706331.3846454
ACGGGGC1307.2759576E-1231.36466616
ACAGGCC1307.2759576E-1231.3646661
CAACGCA23800.031.1230625
Produced by FastQC (version 0.11.5)