FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834045
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33080.3966212854222494No Hit
GTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACCC25600.30693787505470327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25180.30190217554208704No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24310.2914710836945249No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23740.28463692007026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22350.26797115263564913No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACCCTGCTGATTTTCAGTGTGAATGCAGTGCCTGACCCACTGCCG19990.2396753172790437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19760.2369176723078491No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19440.23308094886966532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18990.22768555653471934No Hit
GGCACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17910.21473661493084906No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17590.21089989149266525No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17020.20406572786840038No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16400.1966320762069193No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC16290.1953132025250436No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16100.19303514798362198No Hit
GCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTAGTCAAAGTCCCGTA15760.1889586293305517No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15420.18488211067748142No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15410.18476221307003818No Hit
GGCCAAAAGTGTACGGCCAGTGTGTGCCTTGCATGCAGTAATAAACCCCA15340.18392292981793548No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14590.17493060925969223No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14330.17181327146616787No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14000.16785665042054085No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA13910.16677757195355167No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13710.1643796198046868No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13610.16318064373025434No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13460.1613821796186057No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13080.15682607053576247No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13030.15622658249854626No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12970.15550719685388678No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGCATTCACACTGAA12920.15490770881667054No Hit
GGGTCAGGCACTGCATTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGA12620.15131078059337327No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC12500.14987200930405434No Hit
GTCCAAGGCTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC12470.1495123164817246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12270.14711436433285974No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11920.14291794807234623No Hit
GTTTATTACTGCATGCAAGGCACACACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA11780.1412393815681408No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11570.1387215318118327No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGA11440.13716286291507052No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11200.1342853203364327No Hit
GCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGT10970.1315276753652381No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC10920.1309281873280219No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10860.1302088016833624No Hit
GTCTCCACTCTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTAGTCAAAGTCCCGTATC10860.1302088016833624No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10600.12709146388983808No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10280.1232547404516543No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10160.12181596916233535No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9860.11821904093903807No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9820.11773945050926508No Hit
GCATTCACACTGAAAATCAGCAGGGTGGAGGCTGAAGATGTTGGGGTTTATTACTGCATGCAAGGCACACACTGG9810.11761955290182184No Hit
GTATTACTGTGGAAAAGATGTGTTGCCTGGAGGTCTGGACGTCTGGGGCC9810.11761955290182184No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.11438231750085429No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9510.11402262467852455No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9310.11162467252965966No Hit
GTGTACGGCCAGTGTGTGCCTTGCATGCAGTAATAAACCCCAACATCTTC9270.11114508209988669No Hit
CTCCCTGCCCGTCAGCCTTGGACAGCCGGCCTCCATCTCCTGCAGGGCTA9210.11042569645522723No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9110.1092267203807948No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT9020.10814764191380562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9020.10814764191380562No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATGCAGTGGGGATGTTGTGATGACTCAGTCTCCACT9000.10790784669891913No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8950.10730835866170291No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGATTCCATCATAGGAT8860.10622928019471373No Hit
TTATTACTGCATGCAAGGCACACACTGGCCGTACACTTTTGGCCAGGGGA8860.10622928019471373No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8830.105869587372384No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8760.10503030412028128No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8730.10467061129795154No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8560.1026323519714164No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC8550.10251245436397316No Hit
GTCTGAGACCTGAGGATTCGGCCCTGTATTACTGTGGAAAAGATGTGTTG8500.10191296632675695No Hit
GTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT8470.10155327350442722No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8460.10143337589698398No Hit
GTGTGAATGCAGTGCCTGACCCACTGCCGCTGAATCTGTCTGGGACCCCA8430.10107368307465424No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8400.10071399025232451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)