FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208153
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCGCCTCGCAGGCCATTCATCTGCAGATACA53610.44373518916892146No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39110.3237172775302466No Hit
GTCTATTACTGTGCGAGAGGGGGATATCACGTGGATACCGGCATGGGCTA36790.3045144116680586No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAACCA34590.28630479748839754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33520.2774483033191988No Hit
GTCTACACCGTGGCACCCGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTG32930.2725648158801079No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32170.26627422189077044No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26900.22265391883312793No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25660.21239031811368264No Hit
GCCTATACCCTGAAGATTTTGCAGTTTATTACTGTCTACACCGTGGCACC25580.21172815032533132No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24500.2027888851825886No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23940.19815371066412946No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23180.19186311667479203No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22690.18780733897114024No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21720.17977855453738062No Hit
GGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCC21430.1773781963046071No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCTACTTAGCCTGGTATCAGCAAAGGCCTGGCCAGGCTCCCAGGCT21300.17630217364853623No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21200.1754744639130971No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20620.1706737474475501No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20580.17034266355337443No Hit
GTTTATTACTGTCTACACCGTGGCACCCGGCCTCCGTGGACGTTCGGCCA20440.16918386992375967No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19880.16454869540530048No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19680.1628932759344222No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19180.15875472725722653No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19060.15776147557469955No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18700.15478172052711867No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18590.1538712398181356No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG18490.15304353008269647No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18460.15279521716206473No Hit
GTATTGGATGCATTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG18320.15163642353244997No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18260.15113979769118646No Hit
GTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCCA18230.15089148477055472No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18230.15089148477055472No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18140.15014654600865948No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18130.1500637750351156No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17680.14633908122563946No Hit
CTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCA17470.14460089078121727No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATGCATCCAATACCTAC17440.14435257786058553No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17280.14302824228388292No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17090.14145559378654857No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16870.13963463236858245No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC16830.1393035484744068No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16780.13888969360668724No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16740.13855860971251158No Hit
GTGTAGACAGTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGG16630.13764812900352852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16610.1374825870564407No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC15990.13235078669671807No Hit
CAGTAATAGACGGCCGTGTCGTCGCCTCGCAGGCCATTCATCTGCAGATA15440.1277983831518028No Hit
GGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACTTCCTACAGGGCC15220.1259774217338367No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15180.12564633783966106No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15050.12457031518359016No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14990.12407368934232667No Hit
GTCAGAGTGTTAGCGGCTACTTAGCCTGGTATCAGCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA14900.12332875058043144No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14810.12258381181853623No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC14740.12200441500372884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14680.12150778916246534No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG14610.12092839234765795No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCAAGTGTGAGGGGCAGCTGGTGGAGT14520.12018345358576273No Hit
CTGCTACTCTGGCTCCCAGGAACCACCGGAGAAATTGTGTTGCGGCAGTC14280.1181969502207088No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC14190.11745201145881357No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14170.11728646951172574No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14040.11621044685565488No Hit
GAGGAATACTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAGGAACTCAGTGTATCTG13820.11438948543768877No Hit
GGGCTGGAGTGGGTGGCCAACATAAAAGAAGATGGAAGTGAGGAATACTA13670.11314792083453006No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13640.11289960791389833No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC13360.11058202065466873No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13360.11058202065466873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.10876105923670265No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12930.10702286879228046No Hit
CCCTCAGACTCTCCTGTGAAGTTTCTGGATTTACATTTAGTAGGTATTGG12740.10545022029494609No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12700.10511913640077043No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12690.10503636542722652No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12520.10362925887697999No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12420.10280154914154085No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12380.1024704652473652No Hit
GGTGTAGACAGTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACC12350.10222215232673344No Hit
CTCCAGAGACAACGCCAGGAACTCAGTGTATCTGCAGATGAATGGCCTGCGAGGCGACGACACGGCCGTCTATTA12280.10164275551192606No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG12260.10147721356483823No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGCTCCCTGGCCCCAGTAGCCCATGCCGGTATCCA12240.10131167161775038No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12170.100732274802943No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGATCCCTCAGACTCTCCTGTG12120.10031841993522345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)