FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208153
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCGCCTCGCAGGCCATTCATCTGCAGATACA49450.40930246417465344No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40980.33919544958295844No Hit
GTCTATTACTGTGCGAGAGGGGGATATCACGTGGATACCGGCATGGGCTA36240.2999620081231434No Hit
GTCTACACCGTGGCACCCGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTG32010.26494988631406785No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAACCA31290.258990376218906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29900.24748521089630204No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26990.22339885759502318No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26860.22232283493895227No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24800.20527201438890605No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.20403044978574733No Hit
GCCTATACCCTGAAGATTTTGCAGTTTATTACTGTCTACACCGTGGCACC24210.20038852694981513No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23790.19691214606097074No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23280.19269082641023116No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23260.19252528446314332No Hit
GGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCC22380.18524143879127894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22160.18342047737331282No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCTACTTAGCCTGGTATCAGCAAAGGCCTGGCCAGGCTCCCAGGCT21490.17787482214587058No Hit
GTTTATTACTGTCTACACCGTGGCACCCGGCCTCCGTGGACGTTCGGCCA21400.17712988338397537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21220.17564000586018494No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19970.1652936341671957No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19880.16454869540530048No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18970.15701653681280434No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18650.15436786565939908No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATGCATCCAATACCTAC18600.15395401079167953No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18470.15287798813560866No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18360.1519675074266256No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18070.1495671491938521No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18050.14940160724676427No Hit
GTATTGGATGCATTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG17530.14509751662248074No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17500.14484920370184903No Hit
GTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACCCACTGCCA17430.1442698068870416No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17290.14311101325742684No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16880.13971740334212637No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16880.13971740334212637No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG16700.13822752581833594No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16420.13590993855910632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16290.13483391590303548No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16160.13375789324696458No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16140.13359235129987676No Hit
GGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATACTTCCTACAGGGCC15880.131440305987735No Hit
CTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCA15870.1313575350141911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15840.13110922209355935No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15800.13077813819938366No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15790.1306953672258398No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15680.12978488651685674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15570.12887440580787368No Hit
CTGCTACTCTGGCTCCCAGGAACCACCGGAGAAATTGTGTTGCGGCAGTC15560.12879163483432976No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC15260.12630850562801235No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15070.12473585713067799No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14990.12407368934232667No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14680.12150778916246534No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14630.12109393429474578No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCAAGTGTGAGGGGCAGCTGGTGGAGT14490.11993514066513099No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14490.11993514066513099No Hit
CAGTAATAGACGGCCGTGTCGTCGCCTCGCAGGCCATTCATCTGCAGATA14390.11910743092969185No Hit
GTGTAGACAGTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGG14320.11852803411488445No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14310.11844526314134055No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14130.1169553856175501No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC14060.1163759888027427No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13850.11463779835832053No Hit
GGGCTGGAGTGGGTGGCCAACATAAAAGAAGATGGAAGTGAGGAATACTA13830.11447225641123268No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13700.11339623375516181No Hit
GAGGAATACTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACGCCAGGAACTCAGTGTATCTG13680.11323069180807398No Hit
GTCAGAGTGTTAGCGGCTACTTAGCCTGGTATCAGCAAAGGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATA13460.11140973039010786No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG13350.11049924968112483No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGGGGATCCCTCAGACTCTCCTGTG13280.10991985286631742No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13190.1091749141044222No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC13070.10818166242189524No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13050.10801612047480741No Hit
CTCCAGAGACAACGCCAGGAACTCAGTGTATCTGCAGATGAATGGCCTGCGAGGCGACGACACGGCCGTCTATTA12990.10751949463354393No Hit
GGTGTAGACAGTAATAAACTGCAAAATCTTCAGGGTATAGGCTGCTGATGGTGAGAGTGAAGTCTGCCCCAGACC12930.10702286879228046No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12930.10702286879228046No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12900.1067745558716487No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12820.1061123880832974No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12750.10553299126848999No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC12230.10122890064420649No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12200.10098058772357475No Hit
CCCTCAGACTCTCCTGTGAAGTTTCTGGATTTACATTTAGTAGGTATTGG12190.10089781675003083No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12190.10089781675003083No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12180.10081504577648692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)