FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198406
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT43360.3618139428540912No Hit
GTAATAATACTGCTGACAGTAATAAGCTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT41420.34562577290167107No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35820.2988970348946851No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25930.21637074580734741No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA25730.21470186230709795No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24150.20151768265512687No Hit
GTAATACACACCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA22780.19008583067841783No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22510.187832837953081No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT22060.18407785007751964No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA20240.16889101022524922No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGCTTATTACTGTCAGCAGTATTATTACTGG19450.1622989203992637No Hit
GGAGAAATAGTGATGACGCAGTCGCCAGCCACCCTGTCTGTGTCTCCAGG19280.16088036942405162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19250.1606300368990142No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT18710.15612405144834055No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15870.13242590574479768No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15860.13234246156978519No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAGATCTGGGCAGGCTCCCAGGCT15740.1313411314696355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15710.13109079894459807No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCAGAGATCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA15550.12975569214439847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15470.12908813874429867No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14770.12324704649342544No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA14410.12024305619297633No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTAATAATACTG14350.11974239114290149No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14320.11949205861786408No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14200.11849072851771436No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13970.11657151249242743No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCC13750.114735740642153No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13670.1140681872420532No Hit
CAGTAATACACACCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATA13640.11381785471701576No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13550.1130668571419035No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13500.11264963626684112No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13380.11164830616669141No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGATCTCTGCTGGTACCAGGC13130.10956220179137956No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12930.10789331829113005No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12660.10564032556579324No Hit
GCTTATTACTGTCAGCAGTATTATTACTGGCCTCCGTGGACGTTCGGCCA12480.10413833041556869No Hit
GATCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCC12300.10263633526534413No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12230.10205222604025681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG150.002286611169.51884545
GGTATCA13400.042.5115931
CGTAATT750.002013414236.3323270
CGTATGC1151.8189894E-1236.03640741
TCGTATG1151.8189894E-1235.99052440
GTATCAA34500.035.5788881
TATCAAC38550.031.6651122
ATGCCGT1450.031.11969244
ATCAACG40100.030.4411493
TCAACGC40450.030.2615784
CAACGCA40700.030.0756975
AACGCAG41400.029.7322166
TCTGGAG10750.029.15035470
ATCTCGT1202.8612703E-928.57988737
ACGCAGA44700.027.4590727
TCTCGCG8000.027.2492470
ACGAGAC1504.3655746E-1127.1377722
CCCACGA1750.027.13097619
GTTCGCC500.0084147927.1275776
CGCAGAG45050.027.0952078
Produced by FastQC (version 0.11.5)