FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198406
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTACTGGCCTCCGTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT44140.3683225885050642No Hit
GTAATAATACTGCTGACAGTAATAAGCTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT41820.34896353990217005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33460.279204209591741No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA23400.19525936952919126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22000.18357718502744477No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT20890.17431488160106007No Hit
GTAATACACACCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA20880.1742314374260476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20230.16880756605023672No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19750.16480224564963794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18990.15846048834868987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18550.15478894464814097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18430.15378761454799125No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGCTTATTACTGTCAGCAGTATTATTACTGG18350.15312006114789145No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT16630.13876766304574578No Hit
GGAGAAATAGTGATGACGCAGTCGCCAGCCACCCTGTCTGTGTCTCCAGG16560.13818355382065844No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16120.13451201012010955No Hit
GTGTTAGCACCAACTTAGCCTGGTACCAGCAGAGATCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA14850.12391459989352523No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14510.12107749794310109No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTCCACGGAGGCCAGTAATAATACTG14370.11990927949292644No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT13920.11615429161736507No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTACCAGCAGAGATCTGGGCAGGCTCCCAGGCT13890.11590395909232765No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13840.11548673821726527No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13840.11548673821726527No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13490.11256619209182865No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGCCTGGTA13090.10922842509132964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13070.10906153674130468No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12990.1083939833412049No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12970.10822709499117995No Hit
CAGTAATACACACCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATA12750.1063913231409055No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12570.10488932799068094No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCC12500.10430521876559364No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12330.10288666779038155No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12330.10288666779038155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT200.00647364653.45558
AAAGTCG200.007094865752.23073646
CGCCAAT306.901119E-445.17378214
GGTATCA14100.041.3723641
GTATCAA34700.034.697641
TATCAAC38500.030.818312
TCAACGC38700.030.3759564
ATCAACG38900.030.2286113
CAACGCA39400.029.8362855
AACGCAG40600.029.3716346
GACGAGA1454.319974E-529.07382670
ACGCAGA42350.028.157937
CGCAGAG42250.028.1443928
AGAGTAC43050.027.46055811
CCTAACG500.00842609127.1201132
GCAGAGT44200.026.8260829
TCTGGAG13800.026.47544370
TTAACGG1209.24756E-825.65650735
GACAGTA13200.024.38764615
CAGAGTA48500.024.37580310
Produced by FastQC (version 0.11.5)