FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543432
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC74920.48541173177697494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74790.4845694530112114No Hit
CTTTTATACAGCTACGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA63910.4140771993842295No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC56270.36457712422704724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51640.33457904203100625No Hit
CTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCT51060.3308211829222149No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49920.3234350460532113No Hit
GCTTTGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGTAGCTGTATAAAAGACT49130.3183165827843404No Hit
ATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAA48210.31235584074970585No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47360.3068486334350979No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGGTGATGGTGGTCCTCTCGCCCAGAGACACAGCCAGGGACTCTGGAGA45490.2947327773429604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG45090.29214115037138016No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT44410.28773538451969377No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43610.28255213057653333No Hit
GTTTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAA41380.26810381020997365No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAGCTACGA41260.26732632211849955No Hit
AACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAA39690.2571541862550472No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39370.255080884677783No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACTTTGGTCCCGGGGCCGAAGGTG39350.254951303329204No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39080.25320195512338733No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38530.24963846803746453No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38360.24853702657454296No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38050.2465285156715683No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36890.23901279745398565No Hit
GTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCACTCTCAC36010.23331121811650918No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35930.23279289272219317No Hit
GACTAGCATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT34990.22670256933897964No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT34620.22430531439026793No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA34580.2240461516931099No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34060.2206770366300556No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACTTTGGTCC33860.21938122314426553No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTG33440.2166600148241063No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31980.20720057637783848No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGTAGCTG31720.20551601884631135No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAATAGATGAGCAGCTTTGGAGGCTGTCCTGGTTTCTGCTGGTA31340.20305397322331015No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT29210.1892535595996455No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28710.1860140258851702No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28530.1848477937479591No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG27860.18050681857056222No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27390.17746165687895546No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTG27080.1754531459759808No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26900.1742869138387697No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26880.1741573324901907No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26040.16871491584987225No Hit
GGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC25990.1683909624784247No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23750.15387785143757549No Hit
GTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAGCTACGACAATAAGAACTA23520.15238766592891687No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATA23050.1493425042373101No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTT22630.14662129591715087No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22300.1444832036655972No Hit
CATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCA22130.1433817622026756No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22080.14305780883122807No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21860.14163241399685894No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21860.14163241399685894No Hit
CTTTGGTCCCGGGGCCGAAGGTGGGAGGCAGACTAGCATATTGCTGACAGTAATAAACTGCCACATCTTCAGC21790.1411788792768324No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21690.14053097253393737No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21530.13949432174530527No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATC21210.13742102016804109No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21200.1373562294937516No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21140.13696748544801454No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGA21080.13657874140227752No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20930.13560688128793494No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20860.1351533465679084No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20750.13444064915072385No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20480.1326913009449072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20480.1326913009449072No Hit
CCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAG20470.1326265102706177No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCAC20340.1317842315048541No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTGCCTC20150.13055320869335352No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20080.13009967397332697No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20060.12997009262474798No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCAAAGCTGCTCATC20040.12984051127616897No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19880.12880386048753686No Hit
GCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCAT19880.12880386048753686No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19740.12789679104748378No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT19560.12673055891027268No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19270.12485162935587703No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19130.12394455991582395No Hit
CCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCA19060.12349102519579741No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19050.1234262345215079No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19010.1231670718243499No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18880.12232479305858632No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACACGCTGCCAGTGAATCGGTCAGGGACC18840.12206563036142828No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18720.12128814226995423No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18710.12122335159566472No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18660.12089939822421719No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCAAAGCTGC18610.12057544485276968No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18350.11889088732124253No Hit
TCTATTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCACT18300.118566933949795No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18100.1172711204640049No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17470.11318930798376604No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGC17250.11176391314939693No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17220.11156954112652841No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGTCTACGGGGACATCGTGATGA17160.11118079708079137No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16950.10982019292071175No Hit
GCCACAGTTCGTTTGATAGCCACTTTGGTCCCGGGGCCGAAGGTGGGAGG16800.10884833280636917No Hit
ACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAGCTACGACAATAA16550.10722856594913154No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16520.10703419392626304No Hit
GCTAAGTAGTTCTTATTGTCGTAGCTGTATAAAAGACTCTGGCTGGACTT16420.10638628718336797No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16350.10593275246334143No Hit
GCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGG16270.1054144270690254No Hit
ATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCAT16270.1054144270690254No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16140.10457214830326181No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16120.1044425669546828No Hit
ATTCACTGGCAGCGTGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16080.1041834042575248No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC16070.10411861358323529No Hit
GCAGAAACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATT16060.10405382290894578No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16050.10398903223465628No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16010.10372986953749826No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15960.10340591616605072No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15960.10340591616605072No Hit
TTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGA15900.1030171721203137No Hit
GTACCAGCAGAAACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCTGA15730.1019157306573921No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15540.1006847078458915No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15470.10023117312586496No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)