FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543432
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73570.4766649907478917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61250.396842880023221No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57370.37170409839889285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56470.36587293771283735No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC55820.3616615438840195No Hit
CTTTTATACAGCTACGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA53250.3450103405916166No Hit
ATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAA46520.3014062167947794No Hit
GCTTTGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGTAGCTGTATAAAAGACT46370.3004343566804369No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46320.30011040330898936No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46170.2991385431946467No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45700.29609338150304No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA44470.2881241285654308No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43320.28067320102213766No Hit
CTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCT41260.26732632211849955No Hit
GTGCAGCCACAGTTCGTTTGATAGCCACTTTGGTCCCGGGGCCGAAGGTG40500.26240223087249714No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40230.26065288266668046No Hit
GTTTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAA40190.26039371996952243No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC39660.2569598142321787No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39380.2551456753520725No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGGTGATGGTGGTCCTCTCGCCCAGAGACACAGCCAGGGACTCTGGAGA39010.2527484204033608No Hit
GACTAGCATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT38150.24717642241446333No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35650.23097875384208696No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35480.2298773123791654No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT35390.22929419631055986No Hit
GTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAGCTACGA35320.2288406615905333No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34450.22320387292734634No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33940.21989954853858154No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33160.21484587594400012No Hit
GTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCACTCTCAC32010.207394948400707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATAGCCACTTTGGTCC31990.20726536705212797No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT31870.2064878789606539No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGTAGCTG31560.20447936805767924No Hit
AACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAA31360.20318355457188916No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTG29830.1932705814055948No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29570.19158602387406767No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG29320.18996625701683004No Hit
GTCCCAGACACGCTGCCAGTGAATCGGTCAGGGACCCCGGATTCCCGGGT29280.189707094319672No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29190.18912397825106647No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29090.1884760715081714No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28730.1861436072337492No Hit
GAGTGAAATCTGTCCCAGACACGCTGCCAGTGAATCGGTCAGGGACCCCG27970.18121951598774677No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAATAGATGAGCAGCTTTGGAGGCTGTCCTGGTTTCTGCTGGTA27930.18096035329058877No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACACGCTGCCAGTGAATCGGTCAGGGACC26720.17312068170155862No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26260.17014031068424135No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26040.16871491584987225No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTG25980.1683261718041352No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25370.16437394067247538No Hit
GGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC24480.15860757066070938No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24290.15737654784920876No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24120.1562751063862872No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT23420.15173975918602178No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23390.1515453871631533No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23020.14914813221444156No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT22200.14383529692270214No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTGCCTC22110.14325218085409658No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTT22060.14292822748264905No Hit
CTTTGGTCCCGGGGCCGAAGGTGGGAGGCAGACTAGCATATTGCTGACAGTAATAAACTGCCACATCTTCAGC21740.14085492590538487No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21640.14020701916248984No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21400.1386520429795417No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21370.13845767095667316No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGA21140.13696748544801454No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21100.13670832275085654No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21040.13631957870511952No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20840.1350237652193294No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20820.1348941838707504No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20390.13210818487630166No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20180.13074758071622203No Hit
GTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATA20130.1304236273447745No Hit
GTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAGCTACGACAATAAGAACTA19720.1277672096989048No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19020.1232318624986394No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18980.12297269980148139No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18900.12245437440716533No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18650.12083460754992768No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18590.12044586350419068No Hit
GCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCAT18420.11934442204126906No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18280.11843735260121599No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACACGCTGCCAGTGAATCGGT18270.11837256192692648No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18160.11765986450974193No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18080.11714153911542588No Hit
CCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCA17890.11591051630392528No Hit
CATCTATTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCACTGGCAGCGTGTCTGGGACAGATTTCA17720.1148090748410037No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCAC17610.11409637742381912No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17330.11228223854371298No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17310.11215265719513397No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17090.11072726236076484No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17080.11066247168647533No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGTCTACGGGGACATCGTGATGA17050.11046809966360682No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16960.10988498359500126No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16900.10949623954926424No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16770.10865396078350066No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16660.10794126336631611No Hit
CCAGAGTCCCTGGCTGTGTCTCTGGGCGAGAGGACCACCATCACCTGCAAGTCCAGCCAGAGTCTTTTATACAG16650.1078764726920266No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16650.1078764726920266No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCAAAGCTGCTCATCTATTGGGCATCTACCCGGGAATC16320.10573838044047293No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16190.10489610167470935No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16140.10457214830326181No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16080.1041834042575248No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16060.10405382290894578No Hit
TTATTACTGTCAGCAATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGA16000.10366507886320875No Hit
GCCACAGTTCGTTTGATAGCCACTTTGGTCCCGGGGCCGAAGGTGGGAGG15990.10360028818891924No Hit
ATATGCTAGTCTGCCTCCCACCTTCGGCCCCGGGACCAAAGTGGCTATCAAACGAACTGTGGCTGCACCAT15760.1021101026802606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)