FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090962
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGA46800.4289791945090663No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36830.33759196012326737No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35260.3232009914185829No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA32980.3023020050194232No Hit
GTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGGGAACTGTGGCTGCACCAT29980.2748033387047395No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA27790.2547293122950204No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26600.2438215079901958No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26310.2411633035797764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26010.238413436948308No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTTACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGGGAA23920.21925603274907832No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTC21270.1949655441711077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20980.19230733976068828No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGCGAAGTCTGTCCCAGACCCACTGCCA20540.18827420203453465No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20060.18387441542418526No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19530.17901631770859114No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTGGAGCCTGAAGATT19170.1757164777508291No Hit
CTCCTGCAGGGCCAGTCAGATTCTTACCAACAACTACTTAGCCTGGTACC19090.1749831799824375No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18420.16884181117215816No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18330.16801685118271764No Hit
GTGTATTACTGTGCGAAAGGCGGTGGTACCTATGACTACTGGGGCCAGGG17850.16361706457236824No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17590.16123384682509564No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17260.15820899353048043No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16370.15005105585712428No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15960.1462929047941175No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15920.1459262559099217No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15760.14445966037313857No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15480.14189311818376807No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14870.13630172269978238No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTCTTACCAA14450.13245190941572668No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14430.13226858497362878No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCCTTGATCTCCACCTTGGTCC14320.13126030054209037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13660.12521059395285997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13350.12236906510034262No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13210.12108579400565739No Hit
GTCAGATTCTTACCAACAACTACTTAGCCTGGTACCAGCAGAGACCTGGC13180.12081080734251054No Hit
CATCTATGGTTCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCG13100.12007750957411897No Hit
GTGCAGCCACAGTTCCCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12060.11054463858502864No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12050.11045297636397967No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGATATTGTGTTGACGCAGTCTC12000.10999466525873494No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11840.10852806972195181No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11480.10522822976418977No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11360.10412828311160242No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT11300.10357830978530874No Hit
GAGATATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGG11260.10321166090111296No Hit
GGTCTCTGCTGGTACCAGGCTAAGTAGTTGTTGGTAAGAATCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC11050.1012867542590851No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10920.1000951453854488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG150.002599657367.304872
CCTAACG551.7869024E-742.830382
CTAACGC551.795288E-742.804853
CCGTCAT709.801023E-442.06241670
GGTATCA10450.040.2725871
ATAAACG350.001527510638.4370083
TCTGGAG10350.036.9824170
GTATCAA25950.036.197841
AATGGTA5250.033.6499370
TTCCGCT400.002944215733.6262134
AATACTC400.002944877133.6246725
TCCGTCA550.003168327733.1255269
TGTTAGA12500.032.9769370
TATCAAC28450.032.765292
ATCAACG28750.032.521053
TCAACGC29200.032.0139964
CAACGCA29550.031.7471525
TCAGCAT9200.031.2039170
AACGCAG30100.031.1670576
TCTTGCA950.0043867930.99335570
Produced by FastQC (version 0.11.5)