FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113129
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGG58630.5267134357293719No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53830.4835917490245964No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC44820.40264874960584085No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44170.39680935453123584No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41250.3705769951191641No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40480.3636595578769397No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC38960.3500043570870941No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG38330.34434463570709234No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36640.32916220851311934No Hit
CCTCAGGGGTCCCTGACCGAATCTTTGGCTCCAAGTCTGGCTCCTCAGCGTCCCTGGCCATCAGTGGGCTCCAGT35270.316854560432798No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33460.3005940910712056No Hit
GTCCTGGGCCCAGTCTGTTCTGACTCAGTCACCCTCAACGTCTGGGACCC31890.2864897060448519No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG29530.26528821008167064No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCGTAACCATTCAGA29300.26322196259373354No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGACG28640.2572927306718269No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCGTAACCATTCAGAC28400.2551366463365881No Hit
CCCCTGAGGGGCGCTCATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGCTTA27990.2514533355972219No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27590.24785986170515725No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26510.23815748219658278No Hit
GTAATAATGAGCGCCCCTCAGGGGTCCCTGACCGAATCTTTGGCTCCAAG26320.2364505820978521No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGTCACCCTCAACGTCT26100.23447417145721652No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26070.23420466091531167No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26020.23375547667880361No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGGCGTA25260.22692787628388086No Hit
CATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGG24370.21893239687403704No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23910.21479990189816273No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGGAACTGGGAC23870.21444055450895627No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22940.20608572770990605No Hit
GTTCTGACTCAGTCACCCTCAACGTCTGGGACCCCCGGACAGACGGTCAC22620.20321094859625435No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22000.19764106406355417No Hit
CCCCTCAGGGGTCCCTGACCGAATCTTTGGCTCCAAGTCTGGCTCCTCAG21740.19530530603371218No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21610.1941374270187912No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21400.19225085322545726No Hit
TTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGGAA19930.17904483667211976No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG19000.1706900098730695No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18190.16341322524163865No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18080.16242501992132088No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18030.1619758356848128No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT18020.1618859988375112No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17990.16161648829560635No Hit
GTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGCTTACAGAATTACCT17910.1608977935171934No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17720.15919089341846274No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17590.15802301440354172No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17520.1573941564724304No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACTCAGTCAC17510.1573043196251288No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC17510.1573043196251288No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17450.1567652985413191No Hit
GAATTACCTCCGATGTTGGAGTAGGTTCCAGAACACGAGATGGTGACCGT16860.1514649245505238No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16590.14903932967338018No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC16580.14894949282607856No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16550.1486799822841737No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16480.1480511243530624No Hit
GTCTGAATGGTTACGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCA16410.14742226642195108No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGACGCTGAGGAGCCAGACTTGGAGCCAAAGATTCGGTCAGGGACC16350.1468832453381414No Hit
TTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGGAACTG16110.14472716100290262No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTA16040.14409830307179133No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15950.14328977144607677No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15880.14266091351496546No Hit
GTCTACGGTGGGTCCTTCAGTGGTTATTACTGGAGTTGGATCCGGCAGTC15840.142301566125759No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC15610.14023531863782185No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15600.14014548179052025No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15580.139965808095917No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15510.1393369501648057No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15410.13843858169178955No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT15400.13834874484448792No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA15320.137630050066075No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14820.13313820770099422No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC14750.13250934976988293No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14690.13197032868607322No Hit
CCCTCAACGTCTGGGACCCCCGGACAGACGGTCACCATCTCGTGTTCTGG14490.13017359174004092No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14480.1300837548927393No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGCT14390.12927522326702476No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14340.12882603903051668No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14060.12631060730607144No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13480.12110007016257775No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13380.12020170168956158No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG13170.11831512789622765No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13110.11777610681241797No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATGCTGGAGATTGTCCTGAGT13080.11750659627051312No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA12970.11651839095019535No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12910.11597936986638564No Hit
GCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGGAA12870.11562002247717919No Hit
AGTCAGAACAGACTGGGCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATG12860.11553018562987759No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12810.1150810013933695No Hit
CCTGGGAACTGCTGGTACCAGCTTACAGAATTACCTCCGATGTTGGAGTA12790.11490132769876628No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12680.11391312237844849No Hit
TTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTA12530.11256556966892427No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT12530.11256556966892427No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG12480.11211638543241619No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12450.11184687489051133No Hit
GGTTACGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC12360.1110383432647968No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGCTTACAG12130.10897209577685964No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA11970.10753470622003379No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11870.10663633774701765No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11810.10609731666320794No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11770.10573796927400149No Hit
GTCCGGGGGTCCCAGACGTTGAGGGTGACTGAGTCAGAACAGACTGGGCC11740.10546845873209663No Hit
TGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAG11670.10483960080098532No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11610.10430057971717564No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC11540.10367172178606433No Hit
CCTGAGGGGCGCTCATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCC11480.10313270070225465No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11450.1028631901603498No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT11430.10268351646574657No Hit
AGTCTGTTCTGACTCAGTCACCCTCAACGTCTGGGACCCCCGGACAGACG11430.10268351646574657No Hit
GCTGGTACCAGCTTACAGAATTACCTCCGATGTTGGAGTAGGTTCCAGAA11320.10169531114542878No Hit
GGATGACAGTCTGAATGGTTACGCCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC11300.10151563745082555No Hit
ATGTTGGAGTAGGTTCCAGAACACGAGATGGTGACCGTCTGTCCGGGGGT11240.10097661636701587No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11220.10079694267241264No Hit
GGATGAGGCTGATTATTACTGTGCTTCGTGGGATGACAGTCTGAATGGTTACGCCTTCGGAACTGGGACCAAGGT11190.10052743213050779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)