FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308135
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT67330.5147022287455041No Hit
GATTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG65130.49788439266589457No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC57430.4390219663872612No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA57390.4387161875494502No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55090.42113390437531295No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCC52580.4019462823026675No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG52400.4005702775325176No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50020.3823764366827583No Hit
GTAATAATCAGCCTCATCCCCAGACTGGAGCCCACTGATGGCCAGGGAGG47700.36464126408971553No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC45120.3449185290509007No Hit
ACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGC43870.33536294036930436No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC43660.3337576014707962No Hit
GACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGG42480.3247371257553693No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42180.3224437844717862No Hit
GTATTACTTCCGATGTTGTACCAGCTTCCAGAACAAGAGATGGTGACCCT41430.3167104312628284No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCT39240.29996903989267165No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG38220.29217167952848905No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCACTTCCGAAGACATAACCATTCAGGCTGTCATCCCATGCT36090.2758889564150489No Hit
GTCTGGGGATGAGGCTGATTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGAC33960.2596062333016088No Hit
CCCCTGAGGGCCGCTGAATTTCATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGAAACTGCTGGTACCAGTTAA33440.2556311084100647No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG32950.25188531764687894No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32950.25188531764687894No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32800.2507386470050874No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32420.24783374804588212No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31880.2437057337354325No Hit
TCCCCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGGCAGGGACC31830.24332351018816864No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTC29300.22398299869661772No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC28720.21954920554835702No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG28070.21458029943392692No Hit
CCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT27980.213892297048852No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26540.20288425888765305No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26260.20074380702297545No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25480.19478111968565937No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25470.19470467497620658No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24820.18973576886177648No Hit
GAAGTAATACTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCCCAAA24010.1835437473961021No Hit
GGCCATCAGTGGGCTCCAGTCTGGGGATGAGGCTGATTATTACTGTGGAG23510.17972151192346356No Hit
GTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23120.1767401682548055No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22340.1707774809174894No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG22170.1694779208567923No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT22100.1689428078906229No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC22100.1689428078906229No Hit
TGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAAC22020.16833125021500076No Hit
GTTTGGGGGCCGTTCCTGGAAACTGCTGGTACCAGTTAACAGTATTACTT21980.16802547137718965No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGGC21640.16542635125579547No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC21620.16527346183688993No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21500.16435612532345667No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGGGGATG21430.16382101235728727No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC21190.16198633933042078No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21180.16190989462096803No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20420.15610009670255745No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20380.15579431786474637No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT20370.15571787315529362No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA20370.15571787315529362No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20060.1533480871622577No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19890.15204852710156064No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC19770.15113119058812735No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA19680.15044318820305244No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19530.1492965175612609No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19490.14899073872344978No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19460.1487614045950915No Hit
CTCTTGTTCTGGAAGCTGGTACAACATCGGAAGTAATACTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCC19160.14646806331150836No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGAGG18860.14417472202792525No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18850.14409827731847247No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18780.14356316435230307No Hit
GTCATCCCATGCTCCACAGTAATAATCAGCCTCATCCCCAGACTGGAGCC18330.1401231524269284No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT18320.14004670771747565No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG18300.1398938182985701No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG18200.1391293712040424No Hit
GGATGACAGCCTGAATGGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC17520.133931130961254No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17190.13140845554931257No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGGCAGGGACCCCTGAGGGCCGCTG17130.13094978729259596No Hit
GGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGC17080.1305675637453321No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17020.13010889548861546No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16960.12965022723189884No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16890.12911511426572944No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG16880.12903866955627669No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG16850.1288093354279184No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC16600.12689821769159912No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16060.12277020338114951No Hit
CTGTTAACTGGTACCAGCAGTTTCCAGGAACGGCCCCCAAACTCCTCATC16010.12238797983388564No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15960.12200575628662179No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC15940.12185286686771625No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCTGGCCATGCTGGAGATTGTCCTGAGT15820.120935530354283No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15590.11917730203686927No Hit
GGTTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC15520.11864218907069989No Hit
GCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAG15510.11856574436124712No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG15330.11718973959109726No Hit
GTAATACACGGCAGTGTCCTCAGCCTTTAGGCTGCTGATCTGCAGATATG15190.11611951365875846No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA15130.11566084540204184No Hit
GACATAACCATTCAGGCTGTCATCCCATGCTCCACAGTAATAATCAGCCT15050.11504928772641967No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA15040.1149728430169669No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15000.11466706417915583No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14950.11428484063189195No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14940.11420839592243918No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA14910.11397906179408089No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC14840.11344394882791149No Hit
TTATTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAA14600.11160927580104499No Hit
CAGTATTACTTCCGATGTTGTACCAGCTTCCAGAACAAGAGATGGTGACC14390.11000393690253682No Hit
GCCCCCAAACTCCTCATCTATAATGAAATTCAGCGGCCCTCAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGT14340.10962171335527296No Hit
AGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAG14180.10839859800402864No Hit
TTACTGTGGAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAAGTG14080.10763415090950094No Hit
ATGTTGTACCAGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGT14050.10740481678114262No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG13990.106946148524426No Hit
CCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG13910.10633459084880383No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAGCGTCTGGGA13860.10595236730153998No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13850.10587592259208721No Hit
GCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGGGGATGAGG13760.10518792020701227No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13720.1048821413692012No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCTCCCTCAG13680.1045763625313901No Hit
CAGGGGTCCCTGCCCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC13680.1045763625313901No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC13330.10190079770054314No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA13260.10136568473437374No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG13130.10037190351148773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)